{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:17:00Z","timestamp":1787015820273,"version":"build-2736575974"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,4,12]],"date-time":"2017-04-12T00:00:00Z","timestamp":1491955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001778","name":"Deakin University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001778","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Maggot debridement therapy (\n                    <jats:styled-content style=\"fixed-case\">MDT<\/jats:styled-content>\n                    ) refers to the use of blowfly larvae to clean or debride an infected wound. Most commonly, larvae of\n                    <jats:italic>Lucilia sericata<\/jats:italic>\n                    (Meigen) (Diptera: Calliphoridae) are used, and are sterilized prior to use to ensure no further bacterial infections are introduced during treatment. Current methods sterilize eggs from laboratory\u2010reared blowfly colonies, after which sterile early second instar maggots can be provided to hospitals for use in treatment. Maggots not required for treatment are used for colony regeneration, in which sterility is not maintained. The ability to maintain sterility beyond this would allow further research into fly\u2013bacteria interactions and the effects of different bacteria on the blowfly lifecycle. This study aimed to produce a colony of sterile adults, using current egg sterilization practice, but maintaining sterility through to pupation and emergence. The production of a sterile colony allows further research into the impact of bacteria on fly development and survival. Eggs were placed on a sterile food source within autoclaved plant tissue culture containers to allow growth under sterile conditions. Nutrient agar plating of sterilized and non\u2010sterilized eggs, larvae and adults (post\u2010emergence), as well as the pupation medium and feed source in nutrient broth confirmed the aerobic sterility of all samples involved. The lifecycle of\n                    <jats:italic>L. sericata<\/jats:italic>\n                    was successfully completed through pupation to emergence with no effects on lifespan or oviposition by the newly emerged, sterile adult colony.\n                  <\/jats:p>","DOI":"10.1111\/mve.12232","type":"journal-article","created":{"date-parts":[[2017,4,12]],"date-time":"2017-04-12T09:10:18Z","timestamp":1491988218000},"page":"299-305","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["A new method for the production of sterile colonies of\n                    <i>Lucilia sericata<\/i>"],"prefix":"10.1111","volume":"31","author":[{"given":"N. E.","family":"GASZ","sequence":"first","affiliation":[{"name":"Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences Deakin University  Geelong Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. L.","family":"HARVEY","sequence":"additional","affiliation":[{"name":"Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences Deakin University  Geelong Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13355-013-0181-7"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.1998.tb00502.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2009.09530.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/wrr.12408"},{"key":"e_1_2_5_6_1","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1242\/jeb.25.1.86","article-title":"Observations on the development of Lucilia sericata (Mg.) eggs in sheep fleeces","volume":"25","author":"Davies L.","year":"1948","journal-title":"Journal of Experimental Biology"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1603\/ME13070"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0049815"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2915.2005.00577.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anbehav.2016.03.022"},{"key":"e_1_2_5_11_1","first-page":"265","volume-title":"Chemoecology of Insect Eggs and Egg Deposition","author":"McCall P.J.","year":"2002"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.9.2.222"},{"key":"e_1_2_5_13_1","first-page":"185","article-title":"Sterilization of Lucilia cuprina (Wiedemann) maggots used in therapy of intractable wounds","volume":"22","author":"Mohd Mesri S.","year":"2005","journal-title":"Tropical Biomedicine"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-38.2.161"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-481X.2008.00564.x"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1524-475X.2002.10403.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2915.1995.tb00011.x"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.45.1.55"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007485300036907"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1603\/ME09183"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anbehav.2012.09.013"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-8137.2001.00133.x"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0307-6962.2001.00237.x"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0269-283x.2001.00316.x"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3032.2002.00296.x"}],"container-title":["Medical and Veterinary Entomology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmve.12232","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mve.12232","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T14:00:24Z","timestamp":1762264824000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/mve.12232"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,12]]},"references-count":24,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1111\/mve.12232"],"URL":"https:\/\/doi.org\/10.1111\/mve.12232","archive":["Portico"],"relation":{},"ISSN":["0269-283X","1365-2915"],"issn-type":[{"value":"0269-283X","type":"print"},{"value":"1365-2915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,12]]},"assertion":[{"value":"2016-05-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-04-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}