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In another part of the\n C<\/jats:styled-content>\n errado (\n C<\/jats:styled-content>\n entral\u2010\n W<\/jats:styled-content>\n est region of\n B<\/jats:styled-content>\n razil), a milder form of\n SF<\/jats:styled-content>\n has been recorded. However, neither the rickettsia nor the vector involved have been characterized. The aim of the current study was to analyse genetic variation and the presence of rickettsia in\n A. sculptum<\/jats:italic>\n in\n C<\/jats:styled-content>\n errado, from silent areas and with the milder form of\n SF<\/jats:styled-content>\n . Samples were subjected to\n DNA<\/jats:styled-content>\n extraction, amplification and sequencing of 12\n S<\/jats:styled-content>\n r\n DNA<\/jats:styled-content>\n , cytochrome oxidase subunit\n II<\/jats:styled-content>\n and\n D<\/jats:styled-content>\n \u2010loop mitochondrial genes (for tick population analyses), and\n glt<\/jats:italic>\n A<\/jats:styled-content>\n ,\n htr<\/jats:italic>\n A<\/jats:styled-content>\n ,\n omp<\/jats:italic>\n A<\/jats:styled-content>\n and\n gene<\/jats:italic>\n D<\/jats:styled-content>\n (sca4) genes for rickettsia researches. Exclusive haplotypes with low frequencies, high haplotype diversity and low nucleotide diversity, star\u2010shaped networks and significant results in neutrality tests indicate\n A. sculptum<\/jats:italic>\n population expansions in some areas.\n Rickettsia amblyommatis<\/jats:italic>\n ,\n Candidatus<\/jats:italic>\n R<\/jats:styled-content>\n ickettsia andeanae and\n Rickettsia felis<\/jats:italic>\n were detected. The\n A. sculptum<\/jats:italic>\n diversity is not geographically, or biome delimited, pointing to a different potential in vector capacity, possibly associated with differing tick genetic profiles.\n <\/jats:p>","DOI":"10.1111\/mve.12249","type":"journal-article","created":{"date-parts":[[2017,7,28]],"date-time":"2017-07-28T04:37:47Z","timestamp":1501216667000},"page":"427-437","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Amblyomma sculptum<\/i>\n : genetic diversity and rickettsias in the\n B<\/scp>\n razilian\n C<\/scp>\n errado biome"],"prefix":"10.1111","volume":"31","author":[{"given":"K.","family":"BITENCOURTH","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Refer\u00eancia Nacional em Vetores das Riquetsioses Funda\u00e7\u00e3o Oswaldo Cruz Rio de Janeiro Brazil"},{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Biodiversidade e Sa\u00fade Funda\u00e7\u00e3o Oswaldo Cruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"AMORIM","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Refer\u00eancia Nacional em Vetores das Riquetsioses Funda\u00e7\u00e3o Oswaldo Cruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. V.","family":"DE OLIVEIRA","sequence":"additional","affiliation":[{"name":"Secretaria de Vigil\u00e2ncia em Sa\u00fade, Minist\u00e9rio da Sa\u00fade Unidade T\u00e9cnica de Vigil\u00e2ncia de Zoonoses Bras\u00edlia Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. L.","family":"CAETANO","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Refer\u00eancia Nacional em Vetores das Riquetsioses Funda\u00e7\u00e3o Oswaldo Cruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. M.","family":"VOLOCH","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Evolutiva Te\u00f3rica e Aplicada Universidade Federal do Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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