{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T23:38:15Z","timestamp":1769729895132,"version":"3.49.0"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,8,9]],"date-time":"2018-08-09T00:00:00Z","timestamp":1533772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2019,3]]},"abstract":"Abstract<\/jats:title>\n \n The blow fly genus\n Lucilia<\/jats:italic>\n is composed largely of saprophages and facultative myasis agents, including the economically important species\n Lucilia cuprina<\/jats:italic>\n (Wiedemann) (Diptera: Calliphoridae) and\n Lucilia sericata<\/jats:italic>\n (Meigen). Only one species is generally recognized as an obligate agent of myiasis,\n Lucilia bufonivora<\/jats:italic>\n Moniez, and this is an obligate parasite of toads.\n Lucilia silvarum<\/jats:italic>\n (Meigen), a sister species, behaves mainly as a carrion breeder; however, it has also been reported as a facultative parasite of amphibians. Morphologically, these species are almost identical, and historically this has led to misidentification, taxonomic ambiguity and a paucity of studies of\n \n L. bufo<\/jats:italic>\n <\/jats:styled-content>\n nivora<\/jats:italic>\n . In this study, dipterous larvae were analysed from toad myiasis cases from the U.K., The Netherlands and Switzerland, together with adult specimens of fly species implicated in amphibian parasitism:\n \n L. bufo<\/jats:italic>\n <\/jats:styled-content>\n nivora<\/jats:italic>\n ,\n L. silvarum<\/jats:italic>\n and\n \n Lucilia elongata<\/jats:italic>\n <\/jats:styled-content>\n Shannon (from North America). Partial sequences of two genes,\n cox1<\/jats:italic>\n and\n ef1\u03b1<\/jats:italic>\n , were amplified. Seven additional blow fly species were analysed as outgroups. Bayesian inference trees of\n cox1<\/jats:italic>\n ,\n ef1\u03b1<\/jats:italic>\n and a combined\u2010gene dataset were constructed. All larvae isolated from toads were identified as\n \n L. bufo<\/jats:italic>\n <\/jats:styled-content>\n nivora<\/jats:italic>\n and no specimens of\n \n L. silvarum<\/jats:italic>\n <\/jats:styled-content>\n were implicated in amphibian myiasis. This study confirms\n L. silvarum<\/jats:italic>\n and\n \n L. bufo<\/jats:italic>\n <\/jats:styled-content>\n nivora<\/jats:italic>\n as distinct sister species and provides unambiguous molecular identification of\n \n L. bufo<\/jats:italic>\n <\/jats:styled-content>\n nivora<\/jats:italic>\n .\n <\/jats:p>","DOI":"10.1111\/mve.12328","type":"journal-article","created":{"date-parts":[[2018,8,9]],"date-time":"2018-08-09T06:01:19Z","timestamp":1533794479000},"page":"131-139","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The toad fly\n Lucilia bufonivora<\/i>\n : its evolutionary status and molecular identification"],"prefix":"10.1111","volume":"33","author":[{"given":"G.","family":"Arias\u2010Robledo","sequence":"first","affiliation":[{"name":"School of Biological Sciences University of Bristol Bristol U.K."},{"name":"Department of Biosciences University of Exeter Exeter U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Stark","sequence":"additional","affiliation":[{"name":"Reptile, Amphibian and Fish Conservation the Netherlands (RAVON) Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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