{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:10:52Z","timestamp":1790507452935,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,12,5]],"date-time":"2018-12-05T00:00:00Z","timestamp":1543968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000614","name":"Federation of European Microbiological Societies","doi-asserted-by":"publisher","award":["FEMS\u2010RG\u20102017\u20100047"],"award-info":[{"award-number":["FEMS\u2010RG\u20102017\u20100047"]}],"id":[{"id":"10.13039\/501100000614","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["10\u2010IAHU\u201003"],"award-info":[{"award-number":["10\u2010IAHU\u201003"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Flea identification is a significant issue because some species are considered as important vectors of several human pathogens that have emerged or re\u2010emerged recently, such as\n                    <jats:italic>Bartonella henselae<\/jats:italic>\n                    (Rhizobiales: Bartonellaceae) and\n                    <jats:italic>Rickettsia felis<\/jats:italic>\n                    (Rickettsiales: Rickettsiaceae). Matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF MS) has been evaluated in recent years for the identification of multicellular organisms, including arthropods. A preliminary study corroborated the usefulness of this technique for the rapid identification of fleas, creating a preliminary database containing the spectra of five species of flea. However, longterm flea preservation in ethanol did not appear to be an adequate method of storage in the context of specimen identification by MALDI\u2010TOF MS profiling. The goal of the present work was to assess the performance of MALDI\u2010TOF MS in the identification of seven flea species [\n                    <jats:italic>Ctenocephalides felis<\/jats:italic>\n                    (Siphonaptera: Pulicidae),\n                    <jats:italic>Ctenocephalides canis<\/jats:italic>\n                    ,\n                    <jats:italic>Pulex irritans<\/jats:italic>\n                    (Siphonaptera: Pulicidae),\n                    <jats:italic>Archaeopsylla erinacei<\/jats:italic>\n                    (Siphonaptera: Pulicidae),\n                    <jats:italic>Leptopsylla taschenbergi<\/jats:italic>\n                    (Siphonaptera: Ceratophyllidae),\n                    <jats:italic>Stenoponia tripectinata<\/jats:italic>\n                    (Siphonaptera: Stenoponiidae) and\n                    <jats:italic>Nosopsyllus fasciatus<\/jats:italic>\n                    (Siphonaptera: Ceratophyllidae)] collected in the field and stored in ethanol for different periods of time. The results confirmed that MALDI\u2010TOF MS can be used for the identification of wild fleas stored in ethanol. Furthermore, this technique was able to discriminate not only different flea genera, but also the two congeneric species\n                    <jats:italic>C.\u00a0felis<\/jats:italic>\n                    and\n                    <jats:italic>C.\u00a0canis<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/mve.12351","type":"journal-article","created":{"date-parts":[[2018,12,5]],"date-time":"2018-12-05T10:39:25Z","timestamp":1544006365000},"page":"185-194","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry as a useful tool for\u00a0the\u00a0rapid identification of wild flea vectors preserved in alcohol"],"prefix":"10.1111","volume":"33","author":[{"given":"A.","family":"Zurita","sequence":"first","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Pharmacy University of Seville  Seville Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Djeghar","sequence":"additional","affiliation":[{"name":"Laboratoire d'Am\u00e9lioration et D\u00e9veloppement de la Production V\u00e9g\u00e9tale et Animale (LADPVA), Facult\u00e9 des Sciences de la Nature et de la Vie Ferhat Abbas University  S\u00e9tif Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Callej\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Pharmacy University of Seville  Seville Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Cutillas","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Pharmacy University of Seville  Seville Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Parola","sequence":"additional","affiliation":[{"name":"Institut de Recherche pour le D\u00e9veloppement (IRD), Assistance Publique\u2013H\u00f4pitaux de Marseille (AP\u2010HM), Service de Sant\u00e9 des Arm\u00e9es (SSA), Vecteurs\u2013Infections Tropicales et M\u00e9diterran\u00e9ennes (VITROME), Institut Hospitalo\u2010Universitaire (IHU) M\u00e9diterran\u00e9e Infection, Aix Marseille University  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5764-2421","authenticated-orcid":false,"given":"M.","family":"Laroche","sequence":"additional","affiliation":[{"name":"Institut de Recherche pour le D\u00e9veloppement (IRD), Assistance Publique\u2013H\u00f4pitaux de Marseille (AP\u2010HM), Service de Sant\u00e9 des Arm\u00e9es (SSA), Vecteurs\u2013Infections Tropicales et M\u00e9diterran\u00e9ennes (VITROME), Institut 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