{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T13:37:11Z","timestamp":1792503431470,"version":"4.5.0"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,6,7]],"date-time":"2019-06-07T00:00:00Z","timestamp":1559865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003766","name":"Ministerul Educatiei Nationale","doi-asserted-by":"publisher","award":["173006"],"award-info":[{"award-number":["173006"]}],"id":[{"id":"10.13039\/501100003766","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Spirochetes from the\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    sensu lato (s.l.). (Spirochaetales: Spirochaetaceae) species complex, including the causative agents of Lyme borreliosis, have been isolated from ticks, vertebrate reservoirs and humans. Previous analyses based on direct molecular detection in ticks indicated a considerable diversity of\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    s.l. complex in Serbia. The present study aimed (a) to isolate borrelia strains from Serbia; (b) to determine their genotypic characteristics; and (c) to establish a collection of viable\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    s.l. strains for further biological, ecological and genetic studies. For the present study, 231 adult\n                    <jats:italic>Ixodes ricinus<\/jats:italic>\n                    (Ixodida: Ixodidae) ticks from 16 ecologically different localities in Serbia were individually processed to cultivate\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    s.l. This led to the isolation of 36 strains. A\n                    <jats:italic>hbb<\/jats:italic>\n                    gene quantitative real\u2010time polymerase chain reaction (PCR) based on melting temperature determination and\n                    <jats:italic>ospA<\/jats:italic>\n                    gene sequencing were used to genotype the isolated spirochetes. The species identified based on the\n                    <jats:italic>hbb<\/jats:italic>\n                    gene real\u2010time PCR were:\n                    <jats:italic>Borrelia lusitaniae<\/jats:italic>\n                    (44.4%),\n                    <jats:italic>Borrelia afzelii<\/jats:italic>\n                    (36.1%),\n                    <jats:italic>Borrelia garinii<\/jats:italic>\n                    (13.9%) and\n                    <jats:italic>Borrelia valaisiana<\/jats:italic>\n                    (5.6%), whereas the\n                    <jats:italic>osp<\/jats:italic>\n                    A sequence analysis revealed the occurrence of\n                    <jats:italic>Borrelia bavariensis<\/jats:italic>\n                    . This is the first report of the isolation of\n                    <jats:italic>B. lusitaniae<\/jats:italic>\n                    ,\n                    <jats:italic>B. garinii<\/jats:italic>\n                    ,\n                    <jats:italic>B. bavariensis<\/jats:italic>\n                    and\n                    <jats:italic>B.\u00a0valaisiana<\/jats:italic>\n                    strains in Serbia.\n                  <\/jats:p>","DOI":"10.1111\/mve.12392","type":"journal-article","created":{"date-parts":[[2019,6,7]],"date-time":"2019-06-07T05:03:50Z","timestamp":1559883830000},"page":"512-520","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia"],"prefix":"10.1111","volume":"33","author":[{"given":"S.","family":"\u0106aki\u0107","sequence":"first","affiliation":[{"name":"Group for Medical Entomology, Centre of Excellence for Food and Vector Borne Zoonoses, Institute for Medical Research University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Veinovi\u0107","sequence":"additional","affiliation":[{"name":"Group for Medical Entomology, Centre of Excellence for Food and Vector Borne Zoonoses, Institute for Medical Research University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Cerar","sequence":"additional","affiliation":[{"name":"Laboratory for Diagnostics of Borreliosis and Leptospirosis, Institute for Microbiology and Immunology, Faculty of Medicine University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Mihaljica","sequence":"additional","affiliation":[{"name":"Group for Medical Entomology, Centre of Excellence for Food and Vector Borne Zoonoses, Institute for Medical Research University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Sukara","sequence":"additional","affiliation":[{"name":"Group for Medical Entomology, Centre of Excellence for Food and Vector Borne Zoonoses, Institute for Medical Research University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Ru\u017ei\u0107\u2010Sablji\u0107","sequence":"additional","affiliation":[{"name":"Laboratory for Diagnostics of Borreliosis and Leptospirosis, Institute for Microbiology and Immunology, Faculty of Medicine University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Tomanovi\u0107","sequence":"additional","affiliation":[{"name":"Group for Medical Entomology, Centre of Excellence for Food and Vector Borne Zoonoses, Institute for Medical Research University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/mve.12257"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2009.0139"},{"key":"e_1_2_7_4_1","unstructured":"Estrada\u2010Pe\u00f1a A. 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