{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:07:50Z","timestamp":1790748470979,"version":"4.1.0"},"reference-count":14,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,3,11]],"date-time":"2020-03-11T00:00:00Z","timestamp":1583884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2020,9]]},"abstract":"Abstract<\/jats:title>\n \n Four species of the\n Anopheles maculipennis<\/jats:italic>\n complex have previously been recorded in Sweden. A recent addition to the complex is\n Anopheles daciae<\/jats:italic>\n , which is considered to be closely related to, but distinct from\n Anopheles messeae<\/jats:italic>\n . The original designation of\n An. daciae<\/jats:italic>\n was based on five genetic differences (161, 165, 167, 362 and 382) in the second internal transcribed spacer (ITS) 2 of the ribosomal RNA. Further studies have shown that only two nucleotide differences (362 and 382) robustly separate the species. Thirty\u2010three\n An. maculipennis<\/jats:italic>\n complex mosquitoes were collected in the province of Uppland, Sweden. All were\n An. daciae<\/jats:italic>\n but showed double peaks for three variable positions (161, 165 and 167). When cloned, the intra\u2010individual nucleotide variation was almost exclusively fixed with either TTC or AAT, originally diagnostic for\n An. messae<\/jats:italic>\n and\n An. daciae<\/jats:italic>\n , respectively. To further investigate the intra\u2010individual variation, nine\n An. daciae<\/jats:italic>\n and 11\n An. messeae<\/jats:italic>\n were collected in southern Sweden and their ITS2 fragments were amplified and sequenced using Illumina MiSeq sequencing (Illumina, Inc., San Diego, CA, USA). For the diagnostic nucleotide 382 no intra\u2010individual variation could be detected. However, although each\n An. daciae<\/jats:italic>\n specimen carried several ITS2 sequence variants for the four other nucleotides, there was no intra\u2010individual variation in the\n An. messeae<\/jats:italic>\n specimens.\n <\/jats:p>","DOI":"10.1111\/mve.12436","type":"journal-article","created":{"date-parts":[[2020,3,11]],"date-time":"2020-03-11T12:23:28Z","timestamp":1583929408000},"page":"364-368","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Single nucleotide polymorphism analysis of the ITS2 region of two sympatric malaria mosquito species in Sweden:\n Anopheles daciae<\/i>\n and\n Anopheles messeae<\/i>"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1069-6331","authenticated-orcid":false,"given":"T.","family":"Lilja","sequence":"first","affiliation":[{"name":"Department of Microbiology SVA, National Veterinary Institute Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Ekl\u00f6f","sequence":"additional","affiliation":[{"name":"Department of Microbiology SVA, National Veterinary Institute Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. G. T.","family":"Jaenson","sequence":"additional","affiliation":[{"name":"Department of Organismal Biology Uppsala University Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4341-8330","authenticated-orcid":false,"given":"A.","family":"Lindstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Microbiology SVA, National Veterinary Institute Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9909-1859","authenticated-orcid":false,"given":"O.","family":"Terenius","sequence":"additional","affiliation":[{"name":"Department of Ecology Swedish University of Agricultural Sciences (SLU) Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/45.3.337"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/mve.12023"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-41.4.582"},{"key":"e_1_2_6_5_1","first-page":"4","article-title":"Description of the new species Anopheles artemievi sp. n. 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