{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T00:13:37Z","timestamp":1789776817832,"version":"4.0.1"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T00:00:00Z","timestamp":1596758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006302","name":"Universidad de Alcal\u00e1","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006302","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000831","name":"Natural History Museum","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000831","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    During the larval stage, oestrid flies (Diptera: Oestridae) are obligate parasites, whereas during the adult stage they are free\u2010living and do not feed. Like other cyclorrhaphous flies, oestrids undergo metamorphosis inside an opaque puparium, formed by the contracted and hardened cuticle of the third\u2010instar larva. The present study documents the internal morphological changes taking place during metamorphosis of the sheep nasal bot fly,\n                    <jats:italic>Oestrus ovis<\/jats:italic>\n                    L., using non\u2010invasive, micro\u2010CT\u2010based virtual histology and provides quantitative data of volumetric changes in specific organs. Virtual histological sections allowed visualisation of the progression and completion of the apolyses, which delimit the different intra\u2010puparial stages, and the connection to the tracheal system of a large gas bubble, which plays an essential role during early metamorphosis. Overall, our results show that the sequence of morphological and volumetric changes in tissues and organs is similar to those found in other cyclorrhaphous flies, but they also reveal developmental differences that result in an adult vestigial digestive tract. Future studies could develop non\u2010invasive, reliable methods for aging the intra\u2010puparial forms of different oestrid species of veterinary importance, based on both qualitative and quantitative markers, thus improving our knowledge of their development and the efficiency of control strategies.\n                  <\/jats:p>","DOI":"10.1111\/mve.12465","type":"journal-article","created":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T16:57:27Z","timestamp":1596819447000},"page":"476-487","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Internal morphological changes during metamorphosis in the sheep nasal bot fly,\n                    <scp>\n                      <i>Oestrus ovis<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5626-5441","authenticated-orcid":false,"given":"D.","family":"Mart\u00edn\u2010Vega","sequence":"first","affiliation":[{"name":"Department of Life Sciences University of Alcal\u00e1  Alcal\u00e1 de Henares Spain"},{"name":"Department of Life Sciences Natural History Museum  London U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Clark","sequence":"additional","affiliation":[{"name":"Imaging and Analysis Centre Natural History Museum  London U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. 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