{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T19:13:24Z","timestamp":1779045204905,"version":"3.51.4"},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,9,16]],"date-time":"2020-09-16T00:00:00Z","timestamp":1600214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Spotted fever group (SFG) rickettsiae are obligatory intracellular bacteria that cause disease in humans and other animals. Ixodid ticks are the principal vectors of SFG rickettsiae. The present study aimed to determine the prevalence and species identity of SFG rickettsiae in ticks and horses from urban and rural areas of western Cuba using PCR assays. Tick samples, collected from 79 horses, consisted of 14\n                    <jats:italic>Amblyomma mixtum<\/jats:italic>\n                    adults, 111\n                    <jats:italic>Dermacentor nitens<\/jats:italic>\n                    adults and 19 pools of\n                    <jats:italic>D. nitens<\/jats:italic>\n                    nymphs (2\u20135 individuals\/pool). The PCR results revealed the presence of\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    spp. in 64% of the\n                    <jats:italic>A. mixtum<\/jats:italic>\n                    adults, 16% of the\n                    <jats:italic>D. nitens<\/jats:italic>\n                    adults, and 11% of the pooled samples of\n                    <jats:italic>D. nitens<\/jats:italic>\n                    nymphs. In contrast,\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    spp. was not detected in any of the 200 horse blood samples included in this study. DNA sequence data of the rickettsial 17\u2009kDa antigen gene showed that\n                    <jats:italic>Rickettsia amblyommatis<\/jats:italic>\n                    was present in\n                    <jats:italic>A. mixtum<\/jats:italic>\n                    ; and\n                    <jats:italic>Rickettsia felis<\/jats:italic>\n                    in\n                    <jats:italic>D. nitens<\/jats:italic>\n                    . This is the first report of\n                    <jats:italic>R. felis<\/jats:italic>\n                    in\n                    <jats:italic>D. nitens<\/jats:italic>\n                    in Cuba. The present study extends our knowledge of the potential vector spectrum and distribution of SFG rickettsiae pathogens in western Cuba.\n                  <\/jats:p>","DOI":"10.1111\/mve.12480","type":"journal-article","created":{"date-parts":[[2020,9,16]],"date-time":"2020-09-16T17:54:16Z","timestamp":1600278856000},"page":"207-212","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Molecular detection and identification of spotted fever group rickettsiae in ticks collected from horses in\n                    <scp>Cuba<\/scp>"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7743-7794","authenticated-orcid":false,"given":"A. A.","family":"D\u00edaz\u2010S\u00e1nchez","sequence":"first","affiliation":[{"name":"Centro Nacional de Sanidad Agropecuaria (CENSA), Carretera de Tapaste y Autopista Nacional, Apartado postal 10, San Jos\u00e9 de las Lajas  Mayabeque 32700 Cuba"},{"name":"Department of Biology University of Saskatchewan, 112 Science Place  Saskatoon Saskatchewan S7N 5E2 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. B.","family":"Chilton","sequence":"additional","affiliation":[{"name":"Department of Biology University of Saskatchewan, 112 Science Place  Saskatoon Saskatchewan S7N 5E2 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Roblejo\u2010Arias","sequence":"additional","affiliation":[{"name":"Centro Nacional de Sanidad Agropecuaria (CENSA), Carretera de Tapaste y Autopista Nacional, Apartado postal 10, San Jos\u00e9 de las Lajas  Mayabeque 32700 Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0253-5928","authenticated-orcid":false,"given":"O.","family":"Fonseca\u2010Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Global Health Ume\u00e5 University  Ume\u00e5 901 87 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Marrero\u2010Perera","sequence":"additional","affiliation":[{"name":"Centro Nacional de Sanidad Agropecuaria (CENSA), Carretera de Tapaste y Autopista Nacional, Apartado postal 10, San Jos\u00e9 de las Lajas  Mayabeque 32700 Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. P.","family":"Diyes","sequence":"additional","affiliation":[{"name":"Department of Biology University of Saskatchewan, 112 Science Place  Saskatoon Saskatchewan S7N 5E2 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. E. M.","family":"Yunik","sequence":"additional","affiliation":[{"name":"Department of Biology University of Saskatchewan, 112 Science Place  Saskatoon Saskatchewan S7N 5E2 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Lobo\u2010Rivero","sequence":"additional","affiliation":[{"name":"Centro Nacional de Sanidad Agropecuaria (CENSA), Carretera de Tapaste y Autopista Nacional, Apartado postal 10, San Jos\u00e9 de las Lajas  Mayabeque 32700 Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Corona\u2010Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Centro Nacional de Sanidad Agropecuaria (CENSA), Carretera de Tapaste y Autopista Nacional, Apartado postal 10, San Jos\u00e9 de las Lajas  Mayabeque 32700 Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,16]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2007.0271"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2007.0121"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40475-016-0070-6"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jme\/tjz225"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.29413\/ABS.2018-3.4.25"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-3-26"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762007000700003"},{"key":"e_1_2_4_9_1","doi-asserted-by":"crossref","first-page":"93","DOI":"10.4269\/ajtmh.2004.71.93","article-title":"Prevalence of antibodies to spotted fever group rickettsiae in humans and domestic animals in a Brazilian spotted fever endemic area in the state of S\u00e3o Paulo, Brazil: serological evidence for infection by Rickettsia rickettsii and another spotted fever group Rickettsia","volume":"71","author":"Horta M.C.","year":"2004","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2011.0614"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.1.90-98.2004"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-019-3407-x"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.3958\/059.044.0310"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-811075-1.00002-9"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2016.06.004"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1407.071656"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2015.07.008"},{"key":"e_1_2_4_18_1","volume-title":"PAUP*: phylogentic analysis using parsimony (and other methods) 4.0 Beta","author":"Swofford D.L.","year":"2002"},{"key":"e_1_2_4_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2003.tb07331.x"},{"key":"e_1_2_4_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003487"}],"container-title":["Medical and Veterinary Entomology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mve.12480","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mve.12480","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mve.12480","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T18:18:34Z","timestamp":1762280314000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/mve.12480"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,16]]},"references-count":19,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["10.1111\/mve.12480"],"URL":"https:\/\/doi.org\/10.1111\/mve.12480","archive":["Portico"],"relation":{},"ISSN":["0269-283X","1365-2915"],"issn-type":[{"value":"0269-283X","type":"print"},{"value":"1365-2915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,16]]},"assertion":[{"value":"2020-04-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}