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This study aimed to investigate infection rates of\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    s.l. bacteria in\n                    <jats:italic>I. ricinus<\/jats:italic>\n                    across 24 field sites in England and Wales, focussing on protected recreational areas in National Parks (NPs) and Areas of Outstanding Natural Beauty (AONBs), to provide comparable data across multiple years to assess spatio\u2010temporal changes in\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    s.l. infection. Working with park rangers, questing ticks were collected each spring from 2014 to 2019. A subset of ticks, 4104 nymphs, were analysed using a pan\u2010\n                    <jats:italic>Borrelia<\/jats:italic>\n                    qPCR assay, as well as a\n                    <jats:italic>Borrelia miyamotoi<\/jats:italic>\n                    \u2010specific qPCR, and sequenced to determine\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    s.l. genospecies. Site\u2010specific\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    s.l. infection rates in\n                    <jats:italic>I. ricinus<\/jats:italic>\n                    nymphs varied from 0% to 24%, with overall infection rates ranging from 2.5% to 5.1% across the years. Genospecies composition of sequenced samples was 62.5%\n                    <jats:italic>B. garinii<\/jats:italic>\n                    , 20.3%\n                    <jats:italic>B. valaisiana<\/jats:italic>\n                    and 17.2%\n                    <jats:italic>B. afzelii. Borrelia miyamotoi<\/jats:italic>\n                    was detected in 0.2% of ticks. This study increases our knowledge on\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    s.l.. infection in areas used by the public for outdoor activity across England and Wales, highlighting the spatial and temporal variability which can impact the changing risk to humans from infected tick bites.\n                  <\/jats:p>","DOI":"10.1111\/mve.12503","type":"journal-article","created":{"date-parts":[[2021,1,12]],"date-time":"2021-01-12T18:00:45Z","timestamp":1610474445000},"page":"352-360","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["A nationwide study on\n                    <i>Borrelia burgdorferi<\/i>\n                    s.l. infection rates in questing\n                    <i>Ixodes ricinus<\/i>\n                    : a six\u2010year snapshot study in protected recreational areas in England and Wales"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6814-5669","authenticated-orcid":false,"given":"B.","family":"Cull","sequence":"first","affiliation":[{"name":"Medical Entomology &amp; Zoonoses Ecology, Emergency Response Department Science &amp; Technology Public Health England  Porton Down U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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