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In addition, a comparative test using sandflies with and without eyes evaluated the potential inhibitory effect in the cPCR. An inhibition test was performed using an exogenous DNA added to the qPCR. The genomic DNA quality of each sample was evaluated by cPCR based on the\n                    <jats:italic>cytochrome c oxidase subunit I<\/jats:italic>\n                    (\n                    <jats:italic>cox1<\/jats:italic>\n                    ) gene. The DNA extraction protocols showed the following percentage of amplification: HotShot (91.6% [55\/60]), salting out (71.6% [43\/60]), phenol\u2013chloroform (95% [57\/60]) and kit DNeasy Blood &amp; Tissue Qiagen\u00ae (73.3% [44\/60]). The phenol\u2013chloroform method achieved a significantly higher frequency of\n                    <jats:italic>cox1<\/jats:italic>\n                    gene amplification. The pigment present in the phlebotomine's eyes seems to inhibit cPCR reactions since the frequency of amplification of the\n                    <jats:italic>cox1<\/jats:italic>\n                    gene increased in the sandflies without eyes (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001). The HotShot method showed the highest inhibitory potential. These manual extraction techniques can be an inexpensive and effective alternative to study vector\u2013pathogen interactions.\n                  <\/jats:p>","DOI":"10.1111\/mve.12567","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T06:24:23Z","timestamp":1646115863000},"page":"203-211","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Robust comparative performance of genomic\n                    <scp>DNA<\/scp>\n                    extraction methods from non\u2010engorged phlebotomine sandflies"],"prefix":"10.1111","volume":"36","author":[{"given":"Nath\u00e1lia Alves","family":"Senne","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Veterin\u00e1rias Instituto de Veterin\u00e1ria, Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huarrisson Azevedo","family":"Santos","sequence":"additional","affiliation":[{"name":"Departamento de Epidemiologia e Sa\u00fade P\u00fablica Instituto de Veterin\u00e1ria, Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thamires Rezende","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Veterin\u00e1rias Instituto de Veterin\u00e1ria, Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patr\u00edcia Gonzaga","family":"Paulino","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Veterin\u00e1rias Instituto de Veterin\u00e1ria, Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leo Paulis","family":"Mendon\u00e7a","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Veterin\u00e1rias Instituto de Veterin\u00e1ria, Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haika Vict\u00f3ria Sales","family":"Moreira","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Veterin\u00e1rias Instituto de Veterin\u00e1ria, Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tays Ara\u00fajo","family":"Camilo","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Biol\u00f3gicas, Departamento de Biof\u00edsica Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3698-8340","authenticated-orcid":false,"given":"Isabele","family":"da Costa Angelo","sequence":"additional","affiliation":[{"name":"Departamento de Epidemiologia e Sa\u00fade P\u00fablica Instituto de Veterin\u00e1ria, Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2019.105101"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762002000600022"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.11.022"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jis\/4.1.34"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13592-011-0009-9"},{"key":"e_1_2_10_7_1","first-page":"120","volume-title":"Manual de vigil\u00e2ncia e controle da leishmaniose visceral","author":"Brasil. 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