{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T18:22:24Z","timestamp":1762280544614,"version":"build-2065373602"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T00:00:00Z","timestamp":1687737600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012947","name":"Institut de Recherche pour le D\u00e9veloppement","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012947","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Tsetse flies (\n                    <jats:italic>Glossina<\/jats:italic>\n                    spp.) are major vectors of African trypanosomes, causing either Human or Animal African Trypanosomiasis (HAT or AAT). Several approaches have been developed to control the disease, among which is the anti\u2010vector Sterile Insect Technique. Another approach to anti\u2010vector strategies could consist of controlling the fly's vector competence through hitherto unidentified regulatory factors (genes, proteins, biological pathways, etc.). The present work aims to evaluate the protein abundance in the midgut of wild tsetse flies (\n                    <jats:italic>Glossina palpalis palpalis<\/jats:italic>\n                    ) naturally infected by\n                    <jats:italic>Trypanosoma congolense<\/jats:italic>\n                    s.l. Infected and non\u2010infected flies were sampled in two HAT\/AAT foci in Southern Cameroon. After dissection, the proteomes from the guts of parasite\u2010infected flies were compared to that of uninfected flies to identify quantitative and\/or qualitative changes associated with infection. Among the proteins with increased abundance were fructose\u20101,6\u2010biphosphatase, membrane trafficking proteins, death proteins (or apoptosis proteins) and SERPINs (inhibitor of serine proteases, enzymes considered as trypanosome virulence factors) that displayed the highest increased abundance. The present study, together with previous proteomic and transcriptomic studies on the secretome of trypanosomes from tsetse fly gut extracts, provides data to be explored in further investigations on, for example, mammal host immunisation or on fly vector competence modification via para\u2010transgenic approaches.\n                  <\/jats:p>","DOI":"10.1111\/mve.12676","type":"journal-article","created":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T03:09:43Z","timestamp":1687748983000},"page":"723-736","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protein abundance in the midgut of wild tsetse flies (\n                    <scp>\n                      <i>Glossina palpalis palpalis<\/i>\n                    <\/scp>\n                    ) naturally infected by\n                    <scp>\n                      <i>Trypanosoma congolense<\/i>\n                      s.l.\n                    <\/scp>"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5327-5313","authenticated-orcid":false,"given":"Jean Marc","family":"Tsagmo","sequence":"first","affiliation":[{"name":"INTERTRYP, Institut de Recherche pour le D\u00e9veloppement University of Montpellier  Montpellier France"},{"name":"Faculty of Science University of Yaound\u00e9 I  Yaounde Cameroon"},{"name":"Trypanosome Transmission Group, Trypanosome Cell Biology Unit, Department of Parasites and Insect Vectors and INSERM U1201 Institut Pasteur  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flobert","family":"Njiokou","sequence":"additional","affiliation":[{"name":"Faculty of Science University of Yaound\u00e9 I  Yaounde Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexis","family":"Dziedziech","sequence":"additional","affiliation":[{"name":"Biology of Host\u2010Parasite Interactions Unit, Department of Parasites and Insect Vectors Institut Pasteur  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerie","family":"Rofidal","sequence":"additional","affiliation":[{"name":"IPSiM, Univ Montpellier, CNRS, INRAE, Institut Agro  Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Hem","sequence":"additional","affiliation":[{"name":"IPSiM, Univ Montpellier, CNRS, INRAE, Institut Agro  Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Geiger","sequence":"additional","affiliation":[{"name":"INTERTRYP, Institut de Recherche pour le D\u00e9veloppement University of Montpellier  Montpellier France"},{"name":"Faculty of Science University of Yaound\u00e9 I  Yaounde Cameroon"},{"name":"Center for Research on Filariasis and Other Tropical Diseases (CRFilMT)  Yaounde Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,6,26]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2303"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(03)53002-0"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2017.1331833"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.1100"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2020.104636"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800417"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00000831"},{"key":"e_1_2_11_9_1","unstructured":"Conroy R.M.(2018)The RCSI Sample size handbook.https:\/\/doi.org\/10.13140\/RG.2.2.30497.51043"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1511"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2010.06.002"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001281"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.112.17.2799"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00444"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-005-4525-6"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msl135"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2004.4.230"},{"key":"e_1_2_11_18_1","article-title":"RNA\u2010seq de novo assembly reveals differential gene expression in Glossina palpalis gambiensis infected with Trypanosoma brucei gambiense vs. non\u2010infected and self\u2010cured flies","volume":"6","author":"Hamidou Soumana I.","year":"2015","journal-title":"de novo"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2014.03.005"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00609"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2002094345"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2838"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20145760"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_11_25_1","first-page":"235","article-title":"Description du pi\u00e8ge monoconique utilis\u00e9 pour l\u2019\u00e9limination des glossines en R\u00e9publique Populaire du Congo","volume":"19","author":"Lancien J.","year":"1981","journal-title":"Ent. M\u00e9d. Parasitol."},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-016-3283-0"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2005.04.003"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2004.04.011"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003448"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-5-109"},{"key":"e_1_2_11_31_1","first-page":"55","article-title":"Une nouvelle technique de dissection rapide des glandes salivaires et de l\u2019intestin de glossines","author":"Penchenier L.","year":"1981","journal-title":"Ent. M\u00e9d. Parasitology"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2004.05.001"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2012193217"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2007.09.005"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1099"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00876"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-019-3402-2"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3901"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002857"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00560-13"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-2205-3"},{"key":"e_1_2_11_42_1","first-page":"209","volume-title":"World Health Organization","author":"World Health Organization","year":"2006"}],"container-title":["Medical and Veterinary Entomology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mve.12676","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T18:17:50Z","timestamp":1762280270000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/mve.12676"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,26]]},"references-count":41,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1111\/mve.12676"],"URL":"https:\/\/doi.org\/10.1111\/mve.12676","archive":["Portico"],"relation":{},"ISSN":["0269-283X","1365-2915"],"issn-type":[{"type":"print","value":"0269-283X"},{"type":"electronic","value":"1365-2915"}],"subject":[],"published":{"date-parts":[[2023,6,26]]},"assertion":[{"value":"2022-12-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}