{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T18:23:13Z","timestamp":1762280593988,"version":"build-2065373602"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,7,7]],"date-time":"2023-07-07T00:00:00Z","timestamp":1688688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000270","name":"Natural Environment Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000270","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In vertebrates, enzymes responsible for DNA methylation, one of the epigenetic mechanisms, are encoded by genes falling into the cytosine methyltransferases genes family (\n                    <jats:italic>Dnmt1<\/jats:italic>\n                    ,\n                    <jats:italic>Dnmt3a<\/jats:italic>\n                    ,\n                    <jats:italic>b<\/jats:italic>\n                    and\n                    <jats:italic>Dnmt3L<\/jats:italic>\n                    ). However, in Diptera, only the methyltransferase\n                    <jats:italic>Dnmt2<\/jats:italic>\n                    was found, suggesting that DNA methylation might act differently for species in this order. Moreover, genes involved in epigenetic dynamics, such as\n                    <jats:italic>Ten\u2010eleven Translocation dioxygenases<\/jats:italic>\n                    (\n                    <jats:italic>TET<\/jats:italic>\n                    ) and\n                    <jats:italic>Methyl\u2010CpG\u2010binding domain<\/jats:italic>\n                    (\n                    <jats:italic>MBDs<\/jats:italic>\n                    ), present in vertebrates, might play a role in insects. This work aimed at investigating nucleic acids methylation in the malaria vector\n                    <jats:italic>Anopheles gambiae<\/jats:italic>\n                    (Diptera: Culicidae) by analysing the expression of\n                    <jats:italic>Dnmt2<\/jats:italic>\n                    ,\n                    <jats:italic>TET2<\/jats:italic>\n                    and\n                    <jats:italic>MBDs<\/jats:italic>\n                    genes using quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) at pre\u2010immature stages and in reproductive tissues of adult mosquitoes. In addition, the effect of two DNA methylation inhibitors on larval survival was evaluated. The qPCR results showed an overall low expression of\n                    <jats:italic>Dnmt2<\/jats:italic>\n                    at all developmental stages and in adult reproductive tissues. In contrast,\n                    <jats:italic>MBD<\/jats:italic>\n                    and\n                    <jats:italic>TET2<\/jats:italic>\n                    showed an overall higher expression. In adult mosquito reproductive tissues, the expression level of the three genes in males' testes was significantly higher than that in females' ovaries. The chemical treatments did not affect larval survival. The findings suggest that mechanisms other than DNA methylation underlie epigenetic regulation in\n                    <jats:italic>An. gambiae<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/mve.12681","type":"journal-article","created":{"date-parts":[[2023,7,7]],"date-time":"2023-07-07T05:55:34Z","timestamp":1688709334000},"page":"754-766","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Relative expression of key genes involved in nucleic acids methylation in\n                    <i>Anopheles gambiae<\/i>\n                    sensu stricto"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1571-1265","authenticated-orcid":false,"given":"Oswald Y.","family":"Djihinto","sequence":"first","affiliation":[{"name":"Tropical Infectious Diseases Research Centre (TIDRC) University of Abomey\u2010Calavi  Cotonou 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University of Abomey\u2010Calavi  Cotonou Benin"},{"name":"Institut R\u00e9gional de Sant\u00e9 Publique (IRSP), University of Abomey\u2010Calavi  Ouidah Benin"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,7,7]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_11_2_1","DOI":"10.1159\/000056881"},{"doi-asserted-by":"publisher","key":"e_1_2_11_3_1","DOI":"10.5772\/65359"},{"doi-asserted-by":"publisher","key":"e_1_2_11_4_1","DOI":"10.1007\/978-1-4939-7481-8_1"},{"key":"e_1_2_11_5_1","first-page":"269","article-title":"Dnmt2 is the Most evolutionary conserved and enigmatic cytosine DNA methyltransferase in eukaryotes","volume":"52","author":"Ashapkin 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