{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:24:22Z","timestamp":1762273462133,"version":"build-2065373602"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T00:00:00Z","timestamp":1696723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Dengue virus (DENV) is an arbovirus that comprises four antigenically different serotypes.\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    (Diptera: Culicidae) acts as the principal vector for DENV transmission, and vector control is crucial for dengue fever epidemic management. To design effective vector control strategies, a comprehensive understanding of the insect vector and virus interaction is required. Female\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    ingests DENV during the acquisition of a blood meal from an infected human. DENV enters the insect midgut, replicates inside it and reaches the salivary gland for transmitting DENV to healthy humans during the subsequent feeding cycles. DENV must interact with the proteins present in the midgut and salivary glands to gain entry and accomplish successful replication and transmission.\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    midgut cDNA library was prepared, and yeast two\u2010hybrid screening was performed against the envelope protein domain III (EDIII) protein of DENV\u20102. The polyubiquitin protein was selected from the various candidate proteins for subsequent analysis. Polyubiquitin gene was amplified, and the protein was purified in a heterologous expression system for in vitro interaction studies. In vitro pull\u2010down assay presented a clear interaction between polyubiquitin protein and EDIII. To further confirm this interaction, a dot blot assay was employed, and polyubiquitin protein was found to interact with DENV particles. Our results enable us to suggest that polyubiquitin plays an important role in DENV infection within mosquitoes.\n                  <\/jats:p>","DOI":"10.1111\/mve.12696","type":"journal-article","created":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T19:58:55Z","timestamp":1697054335000},"page":"48-58","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Polyubiquitin protein of\n                    <i>Aedes aegypti<\/i>\n                    as an interacting partner of dengue virus envelope protein"],"prefix":"10.1111","volume":"38","author":[{"given":"Karuna","family":"Yadav","sequence":"first","affiliation":[{"name":"Gut Biology Laboratory, Department of Zoology University of Delhi  Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gunjan Kumar","family":"Saurav","sequence":"additional","affiliation":[{"name":"Gut Biology Laboratory, Department of Zoology University of Delhi  Delhi India"},{"name":"Department of Zoology Rajiv Gandhi University  Doimukh Arunachal Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vipin Singh","family":"Rana","sequence":"additional","affiliation":[{"name":"Gut Biology Laboratory, Department of Zoology University of Delhi  Delhi India"},{"name":"Department of Veterinary Medicine University of Maryland  College Park Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nitish","family":"Rawat","sequence":"additional","affiliation":[{"name":"Gut Biology Laboratory, Department of Zoology University of Delhi  Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Anjali","sequence":"additional","affiliation":[{"name":"Gut Biology Laboratory, Department of Zoology University of Delhi  Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohit","family":"Jamwal","sequence":"additional","affiliation":[{"name":"Gut Biology Laboratory, Department of Zoology University of Delhi  Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5135-6654","authenticated-orcid":false,"given":"Om Prakash","family":"Singh","sequence":"additional","affiliation":[{"name":"National Institute of Malaria Research  Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anannya","family":"Bandyopadhyay","sequence":"additional","affiliation":[{"name":"Protein Homeostasis Laboratory, Department of Zoology University of Delhi  Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1476-8497","authenticated-orcid":false,"given":"Raman","family":"Rajagopal","sequence":"additional","affiliation":[{"name":"Gut Biology Laboratory, Department of Zoology University of Delhi  Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,10,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/v10070367"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.14.5509"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2010.01005.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.1996.tb00045.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2002.67.85"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12060"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.12.8765-8772.1996"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00301072"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.108.135814"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004941"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000901"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuu004"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.04.013"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.3.480"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2460"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61687-0"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-008045382-8.00697-3"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.2149\/tmh.2011-S03"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.21117"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00660-8"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.62.081307.163005"},{"key":"e_1_2_9_24_1","first-page":"1101","article-title":"The viruses and their replication","author":"Lindenbach B.D.","year":"2007","journal-title":"Fields Virology"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01348-16"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003931"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005202"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jctc.1c00302"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2007.05998.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.2.1223-1231.2005"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btn322"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/875958"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10327-009-0147-3"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12538"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13200"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1626"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-019-01657-z"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.04.034"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/v6125028"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.6422"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/v13030369"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/v3122374"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes10060412"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2004.06.019"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1089\/10665270050081478"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12192-017-0860-3"}],"container-title":["Medical and Veterinary Entomology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mve.12696","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:12:50Z","timestamp":1762272770000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/mve.12696"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,8]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1111\/mve.12696"],"URL":"https:\/\/doi.org\/10.1111\/mve.12696","archive":["Portico"],"relation":{},"ISSN":["0269-283X","1365-2915"],"issn-type":[{"type":"print","value":"0269-283X"},{"type":"electronic","value":"1365-2915"}],"subject":[],"published":{"date-parts":[[2023,10,8]]},"assertion":[{"value":"2023-06-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}