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Insecticide\u2010based preventative measures are a key component of vector\u2010borne disease control programmes. However, widespread insecticide resistance threatens the effectiveness of current control strategies. Sarolaner, an isoxazoline insecticide, offers a novel mode of action and is primarily used for controlling ticks, fleas and mites in companion animals. This study evaluates the insecticidal efficacy of sarolaner against both laboratory\u2010susceptible and resistant strains of\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    through various exposure routes. In topical assays, sarolaner outperformed permethrin by &gt;8\u2010fold and &gt;21\u2010fold greater efficacy against resistant strains at 24 and 72\u2009h, respectively. Conversely, it underperformed in susceptible strains by over 8\u2010fold and 2\u2010fold at the same time points. In larval assays, sarolaner exhibited &gt;300\u2010fold greater toxicity than spinosad at 24 and 48\u2009h for both susceptible and resistant strains. Blood\u2010feeding assays showed sarolaner was more toxic than ivermectin by over 17\u2010fold and 10\u2010fold in susceptible and resistant strains, respectively, up to 120\u2009h. While sarolaner was less toxic than dinotefuran in resistant strains through sugar feeding, it was over 3\u2010fold more toxic in susceptible strains. Notably, no cross\u2010resistance was detected with dinotefuran or ivermectin through oral, sugar\u00a0or blood\u2010feeding applications, though slight cross\u2010resistance was observed with permethrin and spinosad. This study highlights sarolaner's potential as an effective adulticide and larvicide against\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    , supporting its further evaluation as a candidate for new chemical formulations.\n                  <\/jats:p>","DOI":"10.1111\/mve.12827","type":"journal-article","created":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T15:24:07Z","timestamp":1752506647000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluating the mosquitocidal potential of the isoxazoline sarolaner against the yellow fever mosquito,\n                    <i>Aedes aegypti<\/i>\n                    (Diptera: Culicidae)"],"prefix":"10.1111","author":[{"given":"Kendra A.","family":"Dagg","sequence":"first","affiliation":[{"name":"Entomology and Nematology Department University of Florida  Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alden S.","family":"Estep","sequence":"additional","affiliation":[{"name":"USDA\u2010ARS Center for Medical, Agricultural, and Veterinary Entomology  Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8144-0897","authenticated-orcid":false,"suffix":"IV","given":"Edwin R.","family":"Burgess","sequence":"additional","affiliation":[{"name":"Entomology and Nematology Department University of Florida  Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/18.2.265a"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-023-04767-0"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.18502\/jad.v17i1.13198"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/3098575"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1603\/ME11025"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-68121-z"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jisesa\/ieaa041"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2015.12.022"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1603\/ME12210"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1603\/ME10021"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008219"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-023-05819-9"},{"key":"e_1_2_10_14_1","unstructured":"ECDC. 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