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Infected dogs serve as the primary domestic reservoir of\n                    <jats:italic>Leishmania<\/jats:italic>\n                    parasites and are often found in close association with various arthropods, such as fleas and ticks. There have been recent reports of\n                    <jats:italic>Leishmania<\/jats:italic>\n                    infections occurring in areas non\u2010endemic for sand fly species, leading to reconsideration of the hypothesis that other arthropods, such as ticks, may also play a significant role in the natural history and epidemiology of leishmaniasis. Here, we used a tick cell line as a tool to study\n                    <jats:italic>Leishmania infantum<\/jats:italic>\n                    and tick interaction. The results showed that\n                    <jats:italic>L. infantum<\/jats:italic>\n                    can bind to and proliferate inside\n                    <jats:italic>Ixodes scapularis<\/jats:italic>\n                    IDE8 tick cells. The infection did reduce tick cell viability and induce reactive oxygen species (ROS) production. Lipid profile analysis showed that the presence of\n                    <jats:italic>L. infantum<\/jats:italic>\n                    increased oxysterol in tick cells and influenced tick cell lipid biosynthesis, since an increase in glycerolipids and esterified cholesterol was observed in infected cells at 48 h. Further experiments are necessary to elucidate whether\n                    <jats:italic>Leishmania<\/jats:italic>\n                    can overcome the various biochemical and tissue barriers within ticks and be transmitted to the host.\n                  <\/jats:p>","DOI":"10.1111\/mve.70038","type":"journal-article","created":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T15:24:08Z","timestamp":1765207448000},"page":"350-359","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unravelling the biochemical aspects of the interaction between ticks and\n                    <i>Leishmania<\/i>\n                    using a tick cell line"],"prefix":"10.1111","volume":"40","author":[{"given":"Beatriz Filgueiras","family":"Silvestre","sequence":"first","affiliation":[{"name":"Departamento de Microbiologia e Imunologia Veterin\u00e1ria, Instituto de Veterin\u00e1ria, Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9453-7241","authenticated-orcid":false,"given":"Karoline dos Anjos","family":"Lima","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica M\u00e9dica Leopoldo de Meis, Universidade Federal do Rio de Janeiro  Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8033-9758","authenticated-orcid":false,"given":"Fernanda de Paula","family":"Pepino","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia e Imunologia Veterin\u00e1ria, Instituto de Veterin\u00e1ria, Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5690-5413","authenticated-orcid":false,"given":"Daniela","family":"Cosentino\u2010Gomes","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Qu\u00edmica, Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5834-141X","authenticated-orcid":false,"given":"Adivaldo","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia e Imunologia Veterin\u00e1ria, Instituto de Veterin\u00e1ria, Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7305-0477","authenticated-orcid":false,"given":"Lesley","family":"Bell\u2010Sakyi","sequence":"additional","affiliation":[{"name":"Department of Infection Biology and Microbiomes Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool  Liverpool UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgia Correa","family":"Atella","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica M\u00e9dica Leopoldo de Meis, Universidade Federal do Rio de Janeiro  Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7085-8649","authenticated-orcid":false,"given":"Lucia H.","family":"Pinto\u2010da\u2010Silva","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia e Imunologia Veterin\u00e1ria, Instituto de Veterin\u00e1ria, Universidade Federal Rural do Rio de Janeiro  Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,8]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2006.03.033"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2018.05.015"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2007.07.009"},{"key":"e_1_2_11_5_1","first-page":"1162","article-title":"La transmission du kala\u2010azar m\u00e9diterran\u00e9en par une tique","volume":"191","author":"Blanc G.","year":"1930","journal-title":"Comptes Rendus de l'Acad\u00e9mie des Sciences de 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