{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T05:19:20Z","timestamp":1787635160514,"version":"build-2736575974"},"reference-count":85,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Vector control is essential for mitigating arbovirus outbreaks that are fuelled by the global spread of medically important mosquito species. Control based on synthetic insecticides can be ineffective due to the increasing evolution of resistance. In response, alternative strategies to insecticides, such as the sterile insect technique, have been developed. These rely on mass production and release of sterile or genetically\u2010modified males to target vector populations. The efficient production of \u2018high\u2010quality\u2019 males is crucial for sustaining these control programmes. Probiotic symbionts, inoculated into larval rearing water, present a potentially valuable tool for insect rearing. Here, we tested the hypothesis that inoculation with\n                    <jats:italic>Asaia<\/jats:italic>\n                    spp. can increase both development rate and adult size of the Yellow Fever mosquito\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    and the common house mosquito,\n                    <jats:italic>Culex pipiens molestus<\/jats:italic>\n                    . Based on previous work, we hypothesized that\n                    <jats:italic>Asaia<\/jats:italic>\n                    inoculation would affect insect development via effects on other components of the insect microbiome. To test this, we conducted 16S rRNA amplicon sequencing on larval and pupal stages. Exposure to\n                    <jats:italic>Asaia<\/jats:italic>\n                    spp. shortened larval development time and increased adult size in\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    and\n                    <jats:italic>Cx. p. molestus<\/jats:italic>\n                    and increased the proportion of insects completing pupation in\n                    <jats:italic>Ae. aegypti.<\/jats:italic>\n                    Effects on adult size were sex\u2010specific but qualitatively consistent across insect species:\n                    <jats:italic>Asaia bogorensis<\/jats:italic>\n                    increased the size of males and females, while\n                    <jats:italic>A. krungthepensis<\/jats:italic>\n                    affected males only. The microbiome analysis was consistent with\n                    <jats:italic>Asaia<\/jats:italic>\n                    affecting insect development via changes in community structure, although inoculation primarily affected the less frequent bacterial taxa. The positive effects of\n                    <jats:italic>Asaia<\/jats:italic>\n                    inoculation on development rate were qualitatively consistent with previous work conducted in a separate insectary, highlighting the reproducibility of\n                    <jats:italic>Asaia<\/jats:italic>\n                    inoculation as a probiotic technique. Overall, our findings suggest that incorporating\n                    <jats:italic>Asaia<\/jats:italic>\n                    as a probiotic symbiont into mass\u2010rearing systems could enhance productivity and yield larger males for release in vector control programs.\n                  <\/jats:p>","DOI":"10.1111\/mve.70109","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T04:39:03Z","timestamp":1787632743000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of\n                    <scp>\n                      <i>A<\/i>\n                    <\/scp>\n                    <i>saia<\/i>\n                    spp. on the development, size and associated microbiomes of two mosquito species of medical importance"],"prefix":"10.1111","author":[{"given":"Alessandro","family":"Roman","sequence":"first","affiliation":[{"name":"Centre for Ecology and Conservation University of Exeter  Penryn UK"},{"name":"Laboratory of Entomology Wageningen University and Research  Wageningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8254-869X","authenticated-orcid":false,"given":"Paula","family":"Luikens","sequence":"additional","affiliation":[{"name":"Laboratory of Entomology Wageningen University and Research  Wageningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constantianus J. M.","family":"Koenraadt","sequence":"additional","affiliation":[{"name":"Laboratory of Entomology Wageningen University and Research  Wageningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3730-0985","authenticated-orcid":false,"given":"Ben","family":"Raymond","sequence":"additional","affiliation":[{"name":"Centre for Ecology and Conservation University of Exeter  Penryn UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1093\/aesa\/saab038","article-title":"The enigmatic Culex pipiens (Diptera: Culicidae) species complex: phylogenetic challenges and opportunities from a notoriously tricky mosquito group","volume":"115","author":"Aardema M.L.","year":"2022","journal-title":"Annals of the Entomological Society of America"},{"key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-020-3879-8","article-title":"Global evaluation of taxonomic relationships and admixture within the Culex pipiens complex of mosquitoes","volume":"13","author":"Aardema M.L.","year":"2020","journal-title":"Parasites & Vectors"},{"key":"e_1_2_11_4_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0011306","article-title":"Variable microbiomes between mosquito lines are maintained across different environments","volume":"17","author":"Accoti A.","year":"2023","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"e_1_2_11_5_1","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1603\/0022-2585-39.4.699","article-title":"Pupal mass and wing length as indicators of fecundity in Aedes albopictus and Aedes geniculatus (Diptera: Culicidae)","volume":"39","author":"Armbruster P.","year":"2002","journal-title":"Journal of Medical Entomology"},{"key":"e_1_2_11_6_1","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1111\/mec.14436","article-title":"Wolbachia infection alters the relative abundance of resident bacteria in adult Aedes aegypti mosquitoes, but not larvae","volume":"27","author":"Audsley M.D.","year":"2018","journal-title":"Molecular Ecology"},{"key":"e_1_2_11_7_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0020699","article-title":"Comparison of life history characteristics of the genetically modified OX513A line and a wild type strain of Aedes aegypti","volume":"6","author":"Bargielowski I.","year":"2011","journal-title":"PLoS One"},{"key":"e_1_2_11_8_1","doi-asserted-by":"crossref","first-page":"4337","DOI":"10.1111\/mec.13762","article-title":"Historical environmental change in Africa drives divergence and admixture of Aedes aegypti mosquitoes: a precursor to successful worldwide colonization?","volume":"25","author":"Bennett K.L.","year":"2016","journal-title":"Molecular Ecology"},{"key":"e_1_2_11_9_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0212520","article-title":"Optimization of irradiation dose to Aedes aegypti and ae. 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