{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T07:46:02Z","timestamp":1791791162383,"version":"4.3.4"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006507","name":"Funda\u00e7\u00e3o Oswaldo Cruz","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Blood feeding is an essential feature to mosquitoes' colony maintenance, once females need blood from host to mature eggs. The once used methods of feeding directly on mammals or other vertebrates have been increasingly substituted for artificial feeders using pre\u2010collected blood, in accordance with good laboratory practices and animal welfare. The artificial feeding method must allow females to lay viable eggs, in rates comparable with those of females fed directly on animals. Here, we tested the efficiency of artificially feeding mosquitoes\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    versus the feeding on anaesthetised mouse in the laboratory. Moreover, we compared reproductive parameters of females fed on rabbit blood treated with three different anticoagulant methods (sodium citrate, heparin and defibrination) to select the best efficiency method. Our results show that artificial feeding was as attractive for females as the anaesthetised mouse, since no significant difference in the percentage of engorged females was found among methods. The average number of eggs laid per female was significantly lower when females fed on blood treated with the anticoagulant sodium citrate, while no difference was found among the other methods. Also, there was no significant difference among methods when egg viability was measured. Overall, our results confirm that different methods used for blood anticoagulation are similar in feeding and reproductive efficiency of mosquitoes\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    , and are equivalent to feeding on anaesthetised mice in the laboratory.\n                  <\/jats:p>","DOI":"10.1111\/mve.70122","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T08:33:39Z","timestamp":1791448419000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effectiveness of different blood anticoagulants for artificial feeding of\n                    <i>Aedes aegypti<\/i>\n                    mosquitoes in the laboratory"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9881-7742","authenticated-orcid":false,"given":"Aline Possati","family":"Cupolillo","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Mosquitos Transmissores de Hematozo\u00e1rios Instituto Oswaldo Cruz, Fiocruz  Rio de Janeiro Brazil"},{"name":"Laborat\u00f3rio de Biologia Molecular de Insetos Instituto Oswaldo Cruz, Fiocruz  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ssica Rodrigues Assun\u00e7\u00e3o","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Insetos Instituto Oswaldo Cruz, Fiocruz  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andresa Borges","family":"de Ara\u00fajo Fonseca","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Insetos Instituto Oswaldo Cruz, Fiocruz  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafaela Vieira","family":"Bruno","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Insetos Instituto Oswaldo Cruz, Fiocruz  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5878-1792","authenticated-orcid":false,"given":"Luciana Ordunha","family":"Araripe","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Insetos Instituto Oswaldo Cruz, Fiocruz  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.52707\/1081-1710-46.2.137"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2022.103448"},{"key":"e_1_2_10_4_1","unstructured":"Araripe L.O.&Cupolillo A.P.(2026)Data source for analysis performed in Cupolillo et al. under review. Zenodo. Available from:https:\/\/doi.org\/10.5281\/zenodo.21007013."},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2015.28"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2024.05.007"},{"key":"e_1_2_10_7_1","volume-title":"Levantamento r\u00e1pido de \u00edndices para Aedes aegypti \u2013 LIRAa \u2013 para vigil\u00e2ncia entomol\u00f3gica do Aedes aegypti no Brasil","author":"Brasil, Minist\u00e9rio da Sa\u00fade","year":"2013"},{"key":"e_1_2_10_8_1","volume-title":"Relat\u00f3rio de uso de animais em Ensino e Pesquisa","author":"Brasil, Minist\u00e9rio de Ci\u00eancia, Tecnologia e Inova\u00e7\u00e3o","year":"2023"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.18474\/0749-8004-24.2.198"},{"key":"e_1_2_10_10_1","volume-title":"The biology of mosquitoes","author":"Clements 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