{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T08:35:12Z","timestamp":1789979712715,"version":"4.0.1"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,8,30]],"date-time":"2012-08-30T00:00:00Z","timestamp":1346284800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Biofilm formation is implicated as a potential virulence factor in <jats:italic>Candida<\/jats:italic> species and carries important clinical repercussions because of their increased resistance to antifungal treatment, ability to withstand host defences and to serve as a reservoir for continuing infections. The present study was undertaken to determine the biofilm production among oral <jats:italic>Candida<\/jats:italic> isolates from HIV\u2010positive and HIV\u2010negative individuals from Pune, India. Biofilm formation was determined using the spectrophotometric or microtitre plate method in 182 <jats:italic>Candida<\/jats:italic> isolates, of which 154 were from HIV\u2010positive and 28 were from HIV\u2010negative individuals. A total of 63.2% of the <jats:italic>Candida<\/jats:italic> isolates were biofilm producers. Significantly increased biofilm forming abilities both qualitatively as well as quantitatively were observed in <jats:italic>Candida<\/jats:italic> isolates from HIV\u2010positive individuals (66.2%) compared to isolates from HIV\u2010negative ones (46.4%), (<jats:italic>P<\/jats:italic>\u2013 0.041). Eighty\u2010one (59.6%) <jats:italic>C. albicans<\/jats:italic> isolates and 34 (73.9%) non \u2013<jats:italic>C. albicans Candida<\/jats:italic> (NCAC) showed biofilm positivity. The NCAC showed significantly greater intensity of biofilm formation compared to the <jats:italic>C. albicans<\/jats:italic>, <jats:italic>P<\/jats:italic>\u2013 0.032. Our results thus show the enhanced biofilm forming abilities of oral <jats:italic>Candida<\/jats:italic> isolates from HIV\u2010infected individuals compared to HIV\u2010uninfected ones and highlight the important role played by biofilm formation in the pathogenesis of NCAC isolates.<\/jats:p>","DOI":"10.1111\/myc.12002","type":"journal-article","created":{"date-parts":[[2012,8,30]],"date-time":"2012-08-30T09:15:06Z","timestamp":1346318106000},"page":"182-186","source":"Crossref","is-referenced-by-count":8,"title":["Biofilm production in oral <i>Candida<\/i> isolates from HIV\u2010positive individuals from Pune, India"],"prefix":"10.1111","volume":"56","author":[{"given":"Arati","family":"Mane","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajinkya","family":"Kulkarni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arun","family":"Risbud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,8,30]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.4.1600-1603.2005"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652006000500004"},{"key":"e_1_2_5_4_2","first-page":"548","article-title":"Adherence to buccal epithelial cells, enzymatic and hemolytic activities of Candida isolates from HIV\u2010infected individuals","volume":"49","author":"Mane A","year":"2011","journal-title":"Med 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