{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:42:49Z","timestamp":1792096969454,"version":"4.4.0"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,3,18]],"date-time":"2013-03-18T00:00:00Z","timestamp":1363564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The strict nutritional requirements of <jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>alassezia<\/jats:italic> species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associated with <jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>alassezia<\/jats:italic> spp. are usually ineffective. The objective of this study was to obtain <jats:italic>in vitro<\/jats:italic> susceptibility profile of 76 clinical isolates of <jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>alassezia<\/jats:italic> species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (<jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>) were obtained by a modified microdilution method based on the Clinical Laboratory Standards Institute reference document M27\u2010A3. The modifications allowed a good growth of all tested species. High <jats:italic>in vitr<\/jats:italic>o antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which presented the highest <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>s and widest range of concentrations. Ketoconazole and itraconazole demonstrated a great activity. Higher <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>s values were obtained with <jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>alassezia furfur<\/jats:italic> indicating a low susceptibility to most of the antifungal agents tested. <jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>alassezia sympodialis<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>alassezia pachydermatis<\/jats:italic> were found to be more\u2010susceptible species than <jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>.\u00a0furfur<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>alassezia globosa<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>alassezia slooffiae<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>alassezia restricta<\/jats:italic>. Topical substances were also active but provide higher <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>s than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treatment regimen.<\/jats:p>","DOI":"10.1111\/myc.12076","type":"journal-article","created":{"date-parts":[[2013,3,18]],"date-time":"2013-03-18T06:15:45Z","timestamp":1363587345000},"page":"571-575","source":"Crossref","is-referenced-by-count":41,"title":["<i>In vitro<\/i> antifungal activity of topical and systemic antifungal drugs against <i><scp>M<\/scp>alassezia<\/i> species"],"prefix":"10.1111","volume":"56","author":[{"given":"Alfonso Javier","family":"Carrillo\u2010Mu\u00f1oz","sequence":"first","affiliation":[{"name":"Dept. 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