{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:51:17Z","timestamp":1790718677273,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,6,4]],"date-time":"2013-06-04T00:00:00Z","timestamp":1370304000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2014,1]]},"abstract":"Summary<\/jats:title>\n \n Onychomycosis is one of the most prevalent dermatophytic diseases. Mycological methods used in the conventional diagnosis may not be optimal. Multiplex (\n MX<\/jats:styled-content>\n )\n PCR<\/jats:styled-content>\n was reported as a reliable alternative. Dermatophyte gene sequence records were used to design a\n MX PCR<\/jats:styled-content>\n for detection and identification of dermatophytes in nail specimens. A\n MX PCR<\/jats:styled-content>\n method based on the amplification of the chitin synthase 1 and internal transcribed spacer genes was developed. The study included 93 strains of dermatophytes and non\u2010dermatophytic fungi, six dermatophytic reference strains and 201 nail specimens from patients with dermatophytic onyxis.\n DNA<\/jats:styled-content>\n extraction directly from nail samples was carried out by using the QIAamp\n DNA<\/jats:styled-content>\n extraction kit (Quiagen). A set of primers was designed and their specificity was assessed.\n MX PCR<\/jats:styled-content>\n detected the causal agent in specimens from which\n \n T<\/jats:styled-content>\n richophyton rubrum\n <\/jats:italic>\n and\n \n T<\/jats:styled-content>\n . interdigitale\n <\/jats:italic>\n grew in culture and also identified a dermatophyte species in an additional 32 specimens that were negative in microscopy and culture. None of the investigated non\u2010dermatophytic strains was positive. Sensitivity of\n MX PCR<\/jats:styled-content>\n was higher as compared to mycological examination (97% vs. 81.1%).\n MX PCR<\/jats:styled-content>\n for direct detection of dermatophytes from nail samples yielded mixed flora in 32.8% of samples.\n MX PCR<\/jats:styled-content>\n proved sensitive and adequate for the diagnosis of dermatophytic onychomycosis. It is much adapted to cases where culture is negative or contaminated by overgrowing moulds, which makes the identification of the causal agent problematic.\n <\/jats:p>","DOI":"10.1111\/myc.12096","type":"journal-article","created":{"date-parts":[[2013,6,4]],"date-time":"2013-06-04T21:44:32Z","timestamp":1370382272000},"page":"19-26","source":"Crossref","is-referenced-by-count":44,"title":["Multiplex\n PCR<\/scp>\n assay for the detection of common dermatophyte nail infections"],"prefix":"10.1111","volume":"57","author":[{"given":"I.","family":"Dhib","sequence":"first","affiliation":[{"name":"Parasitology\u2010Mycology Laboratory Farhat Hached Hospital Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Fathallah","sequence":"additional","affiliation":[{"name":"Parasitology\u2010Mycology Laboratory Farhat Hached Hospital Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Yaacoub","sequence":"additional","affiliation":[{"name":"Parasitology\u2010Mycology Laboratory Farhat Hached Hospital Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Hadj Slama","sequence":"additional","affiliation":[{"name":"Medicine Faculty Immunology and Genetic Laboratory Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. B.","family":"Said","sequence":"additional","affiliation":[{"name":"Parasitology\u2010Mycology Laboratory Farhat Hached Hospital Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Zemni","sequence":"additional","affiliation":[{"name":"Medicine Faculty Immunology and Genetic Laboratory Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,6,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.4.1419-1427.2006"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00100-08"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00829-07"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2009.09291.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46723-0"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2009.01771.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2007.08110.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02367-06"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.2009.00624.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2010.10186.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2007.08100.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00697-08"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02235-07"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2010.01.004"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.10.3617-3622.2001"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.8.2860-2865.2002"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02072-06"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3109\/13693780903531579"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2007.03378.x"},{"key":"e_1_2_7_21_1","first-page":"37","article-title":"Direct detection and differentiation of causative fungi of onychomycosis by multiplex polymerase chain reaction\u2010based assay","volume":"21","author":"Li XF","year":"2010","journal-title":"Eur J Dermatol"},{"key":"e_1_2_7_22_1","article-title":"Detection of Trichophyton rubrum by multiplex PCR and all other dermatophytes from nails","author":"Spiliopoulou A","year":"2011","journal-title":"Eur Soc Clin Microbiol Inf Dis"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-008-9100-9"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2008.01651.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.471-471.2000"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.3109\/13693780903531579"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmyc.12096","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.12096","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T11:35:36Z","timestamp":1696246536000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.12096"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6,4]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1111\/myc.12096"],"URL":"https:\/\/doi.org\/10.1111\/myc.12096","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,4]]}}}