{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T19:27:27Z","timestamp":1789932447537,"version":"4.0.1"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,6,21]],"date-time":"2013-06-21T00:00:00Z","timestamp":1371772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Diagnosis of aspergillosis is often difficult. We compared fungal yields from respiratory specimens using the Health Protection Agency standard culture method (<jats:styled-content style=\"fixed-case\">BSOP<\/jats:styled-content>57), a higher volume undiluted culture method Mycology Reference Centre Manchester (<jats:styled-content style=\"fixed-case\">MRCM<\/jats:styled-content>) and <jats:italic>Aspergillus<\/jats:italic> quantitative real time polymerase chain reaction (q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>). Sputum, bronchial aspirate and bronchoalveolar lavage (<jats:styled-content style=\"fixed-case\">BAL<\/jats:styled-content>) samples (total 23) were collected from aspergillosis patients. One fraction of all samples was cultured using the <jats:styled-content style=\"fixed-case\">MRCM<\/jats:styled-content> method, one <jats:styled-content style=\"fixed-case\">BSOP<\/jats:styled-content>57 and one was used for q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. The recovery rate for fungi was significantly higher by <jats:styled-content style=\"fixed-case\">MRCM<\/jats:styled-content> (87%) than by <jats:styled-content style=\"fixed-case\">BSOP<\/jats:styled-content>57 (8.7%) from all 23 specimens. Sputum samples were 44% positive by <jats:styled-content style=\"fixed-case\">MRCM<\/jats:styled-content> compared to no fungi isolated (0%) by <jats:styled-content style=\"fixed-case\">BSOP<\/jats:styled-content>57. Bronchial aspirates were 75% positive by <jats:styled-content style=\"fixed-case\">MRCM<\/jats:styled-content> and 0% by <jats:styled-content style=\"fixed-case\">BSOP<\/jats:styled-content>57. <jats:styled-content style=\"fixed-case\">BAL<\/jats:styled-content> samples were positive in 20% by <jats:styled-content style=\"fixed-case\">MRCM<\/jats:styled-content> and 10% by <jats:styled-content style=\"fixed-case\">BSOP<\/jats:styled-content>57. q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> was always more sensitive than culture (95.6%) from all samples. In general, over 100 mould colonies (81 <jats:italic>Aspergillus fumigatus<\/jats:italic>) were grown using the <jats:styled-content style=\"fixed-case\">MRCM<\/jats:styled-content> method compared with only one colony from <jats:styled-content style=\"fixed-case\">BSOP<\/jats:styled-content>57. This study provides a reference point for standardisation of respiratory sample processing in diagnostic laboratories. Culture from higher volume undiluted respiratory specimens has a much higher yield for <jats:italic>Aspergillus<\/jats:italic> than <jats:styled-content style=\"fixed-case\">BSOP<\/jats:styled-content>57. q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> is much more sensitive than culture and the current <jats:styled-content style=\"fixed-case\">UK<\/jats:styled-content> method requires revision.<\/jats:p>","DOI":"10.1111\/myc.12103","type":"journal-article","created":{"date-parts":[[2013,6,21]],"date-time":"2013-06-21T09:42:57Z","timestamp":1371807777000},"page":"69-78","source":"Crossref","is-referenced-by-count":75,"title":["Volume dependency for culture of fungi from respiratory secretions and increased sensitivity of <i><scp>A<\/scp>spergillus<\/i> quantitative <scp>PCR<\/scp>"],"prefix":"10.1111","volume":"57","author":[{"given":"Marcin G.","family":"Fraczek","sequence":"first","affiliation":[{"name":"Institute of Inflammation and Repair Education and Research Centre Manchester Academic Health Science Centre (MAHSC) University of Manchester Manchester UK"},{"name":"Mycology Reference Centre Education and Research Centre University Hospital of South Manchester (UHSM) Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie B.","family":"Kirwan","sequence":"additional","affiliation":[{"name":"Institute of Inflammation and Repair Education and Research Centre Manchester Academic Health Science Centre (MAHSC) University of Manchester Manchester UK"},{"name":"National Aspergillosis Centre University Hospital of South Manchester (UHSM) Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline B.","family":"Moore","sequence":"additional","affiliation":[{"name":"Institute of Inflammation and Repair Education and Research Centre Manchester Academic Health Science Centre (MAHSC) University of Manchester Manchester UK"},{"name":"Mycology Reference Centre Education and Research Centre University Hospital of South Manchester (UHSM) Manchester UK"},{"name":"National Aspergillosis Centre University Hospital of South Manchester (UHSM) Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Morris","sequence":"additional","affiliation":[{"name":"Medical Statistics Education and Research Centre University Hospital of South Manchester (UHSM) Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Denning","sequence":"additional","affiliation":[{"name":"Institute of Inflammation and Repair Education and Research Centre Manchester Academic Health Science Centre (MAHSC) University of Manchester Manchester UK"},{"name":"Mycology Reference Centre Education and Research Centre University Hospital of South Manchester (UHSM) Manchester UK"},{"name":"National Aspergillosis Centre University Hospital of South Manchester (UHSM) Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malcolm D.","family":"Richardson","sequence":"additional","affiliation":[{"name":"Institute of Inflammation and Repair Education and Research Centre Manchester Academic Health Science Centre (MAHSC) University of Manchester Manchester UK"},{"name":"Mycology Reference Centre Education and Research Centre University Hospital of South Manchester (UHSM) Manchester UK"},{"name":"National Aspergillosis Centre University Hospital of South Manchester (UHSM) Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,6,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.2.310"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.5.2075-2079.2005"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(05)70238-3"},{"key":"e_1_2_7_5_1","unstructured":"BuckleyM.The fungal kingdom: diverse and essential roles in earth's ecosystem. 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