{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:20:24Z","timestamp":1791202824327,"version":"4.2.0"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,7,15]],"date-time":"2013-07-15T00:00:00Z","timestamp":1373846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The <jats:italic>Cryptococcus neoformans<\/jats:italic>\/<jats:italic>C.\u00a0gattii<\/jats:italic> species complex members are the main agents of systemic cryptococcosis. This disease is believed to be acquired from the environment via fungal cell inhalation. Often, isolates recovered from environmental and clinical sources have proven to be genotypically similar. We assessed the occurrence of <jats:italic>C.\u00a0neoformans<\/jats:italic> and <jats:italic>C.\u00a0gattii<\/jats:italic> in environmental substrates collected in a Portuguese region. Twenty\u2010eight isolates were identified as <jats:italic>C.\u00a0neoformans<\/jats:italic> \u2013 five from decaying <jats:italic>Eucalyptus<\/jats:italic> leaves and 23 from domestic pigeon droppings. The isolates were genotyped using a <jats:italic>URA5<\/jats:italic>\u2010RFLP approach. The <jats:italic>C. neoformans<\/jats:italic> VNIV (53.6%, <jats:italic>n<\/jats:italic>\u00a0=\u00a015) and VNI (32.1%, <jats:italic>n<\/jats:italic>\u00a0=\u00a09) genotypes were abundantly present among environmental isolates. The hybrid VNIII (14.3%, <jats:italic>n<\/jats:italic>\u00a0=\u00a04) genotype was underrepresented and the VNII was not found. <jats:italic>Cryptococcus gattii<\/jats:italic> was also not found although some isolates yielded a positive canavanine\u2013glycine\u2013bromothymol blue test.<\/jats:p>","DOI":"10.1111\/myc.12106","type":"journal-article","created":{"date-parts":[[2013,7,15]],"date-time":"2013-07-15T08:56:42Z","timestamp":1373878602000},"page":"98-104","source":"Crossref","is-referenced-by-count":16,"title":["Genotypic diversity of environmental <i>Cryptococcus neoformans<\/i> isolates from Northern Portugal"],"prefix":"10.1111","volume":"57","author":[{"given":"Ana Sofia","family":"Ferreira","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio Nacional de Investiga\u00e7\u00e3o Veterin\u00e1ria Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria Lisbon Portugal"},{"name":"Microbiology Unit Instituto de Higiene e Medicina Tropical Universidade Nova de Lisboa Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Departamento de Biologia e Ambiente Centro de Investiga\u00e7\u00e3o e de Tecnologias Agroambientais e Biol\u00f3gicas (CITAB) Universidade de Tr\u00e1s\u2010os\u2010Montes e Alto Douro (UTAD) Vila Real Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula","family":"Maduro","sequence":"additional","affiliation":[{"name":"Microbiology Unit Instituto de Higiene e Medicina Tropical Universidade Nova de Lisboa Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In\u00eas","family":"Silva","sequence":"additional","affiliation":[{"name":"Microbiology Unit Instituto de Higiene e Medicina Tropical Universidade Nova de Lisboa Lisbon 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