{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T12:33:21Z","timestamp":1763987601959},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,12,2]],"date-time":"2013-12-02T00:00:00Z","timestamp":1385942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The internal transcribed spacer (ITS) regions of <jats:styled-content style=\"fixed-case\">rDNA<\/jats:styled-content> genes of 49 <jats:italic>Histoplasma capsulatum<\/jats:italic> (48 from clinical samples and one from soil) isolates were examined. Nucleotide sequence heterogeneity within this region was useful for phylogenetic classification of <jats:italic>H.\u00a0capsulatum<\/jats:italic> and species identification. Thus, in 45 of 49 isolates we observed higher percentages of identity in the nucleotide sequences of ITS regions when the isolates studied herein were compared with those reported in our country in the South America B clade. Phylogenetic analyses of <jats:styled-content style=\"fixed-case\">rDNA<\/jats:styled-content> sequences corresponding to the 537 bp of the ITS region obtained from <jats:italic>H.\u00a0capsulatum<\/jats:italic> isolates assigned South America type B clade (45 isolates), North America type 1 and Asia clade (2 isolates each one). <jats:italic>H.\u00a0capsulatum<\/jats:italic> strains isolated from soil and from patients living in Argentina (45 of 49) clustered together with the <jats:italic>H.\u00a0capsulatum<\/jats:italic> isolates of the South America B clade. The high level of genetic similarity among our isolates suggests that almost one genetic population is present in the microenvironment. Isolates described as <jats:italic>H.\u00a0capsulatum<\/jats:italic> var. <jats:italic>capsulatum<\/jats:italic> or var. <jats:italic>farciminosum<\/jats:italic> (2 isolates) did not form a monophyletic group and were found in the Asia clade. Subsequent studies are needed to properly identify these isolates.<\/jats:p>","DOI":"10.1111\/myc.12159","type":"journal-article","created":{"date-parts":[[2013,12,3]],"date-time":"2013-12-03T02:29:19Z","timestamp":1386037759000},"page":"299-306","source":"Crossref","is-referenced-by-count":10,"title":["Genetic diversity of <i>Histoplasma capsulatum<\/i> strains isolated from Argentina based on nucleotide sequence variations in the internal transcribed spacer regions of <scp>rDNA<\/scp>"],"prefix":"10.1111","volume":"57","author":[{"given":"Fernanda","family":"Landaburu","sequence":"first","affiliation":[{"name":"Instituto of Microbiolog\u00eda y Parasitolog\u00eda M\u00e9dica Universidad de Buenos Aires\u2010Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (IMPaM, UBA\u2010CONICET) Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Luj\u00e1n","family":"Cuestas","sequence":"additional","affiliation":[{"name":"Instituto of Microbiolog\u00eda y Parasitolog\u00eda M\u00e9dica Universidad de Buenos Aires\u2010Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (IMPaM, UBA\u2010CONICET) Buenos Aires Argentina"},{"name":"CONICET Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Rubio","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas en Retrovirus y SIDA (INBIRS) Facultad de Medicina Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nahuel Alejandro","family":"El\u00edas","sequence":"additional","affiliation":[{"name":"Instituto of Microbiolog\u00eda y Parasitolog\u00eda M\u00e9dica Universidad de Buenos Aires\u2010Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (IMPaM, UBA\u2010CONICET) Buenos Aires 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