{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T08:50:07Z","timestamp":1784191807789,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,3,21]],"date-time":"2014-03-21T00:00:00Z","timestamp":1395360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Invasive aspergillosis (<jats:styled-content style=\"fixed-case\">IA<\/jats:styled-content>) remains difficult to diagnose in immunocompromised patients, because diagnostic <jats:styled-content style=\"fixed-case\">EORTC<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MSG<\/jats:styled-content> criteria are often not met. As biomarkers might elucidate the pathogen, we analysed the performance of an <jats:italic>Aspergillus <\/jats:italic><jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assay in blood for diagnosis of <jats:styled-content style=\"fixed-case\">IA<\/jats:styled-content> in immunocompromised paediatric patients with suspected infections. Ninety\u2010five haemato\u2010oncological paediatric patients were included over a period of 3\u00a0years, the underlying diseases consisting of acute leukaemia, solid tumours, non\u2010malignant immunocompromising disorders and haematopoietic stem cell transplantation recipients. We retrospectively analysed 253 consecutive episodes of suspected infections. Thirty\u2010eight patients had possible <jats:styled-content style=\"fixed-case\">IA<\/jats:styled-content>, none of the patients fulfilled <jats:styled-content style=\"fixed-case\">EORTC<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MSG<\/jats:styled-content> criteria of probable\/proven <jats:styled-content style=\"fixed-case\">IA<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> positivity was observed in 97\/967 analyses. Sensitivity, specificity, positive and negative predictive value of the <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> per episode were 34%, 78%, 31% and 81% using possible <jats:styled-content style=\"fixed-case\">IA<\/jats:styled-content> as endpoint. Taken together, an undirected blood screening by <jats:italic>Aspergillus<\/jats:italic>\u2010specific <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> is of little diagnostic value in a heterogenous paediatric patient cohort. Harnessing <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> for diagnosis of <jats:styled-content style=\"fixed-case\">IA<\/jats:styled-content> should thus be focused on blood analyses of more homogenous high\u2010risk patients and\/or analyses of bronchoalveolar lavage, tissue or cerebrospinal fluid specimens.<\/jats:p>","DOI":"10.1111\/myc.12192","type":"journal-article","created":{"date-parts":[[2014,3,21]],"date-time":"2014-03-21T09:13:54Z","timestamp":1395393234000},"page":"537-543","source":"Crossref","is-referenced-by-count":21,"title":["Assessment of <i>Aspergillus<\/i>\u2010specific <scp>PCR<\/scp> as a screening method for invasive aspergillosis in paediatric cancer patients and allogeneic haematopoietic stem cell recipients with suspected infections"],"prefix":"10.1111","volume":"57","author":[{"given":"M.","family":"Reinwald","sequence":"first","affiliation":[{"name":"Department of Hematology and Oncology Mannheim University Hospital Mannheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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M.","family":"Konietzka","sequence":"additional","affiliation":[{"name":"Infectious Diseases Research Program Department of Pediatric Hematology and Oncology University Children's Hospital M\u00fcnster M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Kolve","sequence":"additional","affiliation":[{"name":"Central Pharmacy Department University Hospital M\u00fcnster M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Uhlenbrock","sequence":"additional","affiliation":[{"name":"Central Pharmacy Department University Hospital M\u00fcnster M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Ahlke","sequence":"additional","affiliation":[{"name":"Infectious Diseases Research Program Department of Pediatric Hematology and Oncology University Children's Hospital M\u00fcnster M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Hummel","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Mannheim University Hospital Mannheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Spiess","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Mannheim University Hospital Mannheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.\u2010K.","family":"Hofmann","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Mannheim University Hospital Mannheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Buchheidt","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Mannheim University Hospital Mannheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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