{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T02:16:52Z","timestamp":1784081812160,"version":"3.55.0"},"reference-count":28,"publisher":"Wiley","issue":"s3","license":[{"start":{"date-parts":[[2014,9,30]],"date-time":"2014-09-30T00:00:00Z","timestamp":1412035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31140023"],"award-info":[{"award-number":["31140023"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Rhizopus<\/jats:italic> is the most common genus of invasive mucormycosis, whose prognosis and outcome was not improved over the past decades. We studied the apoptotic\u2010like phenotype in <jats:italic>Rhizopus arrhizus<\/jats:italic> exposed to hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) and amphotericin B (<jats:styled-content style=\"fixed-case\">AMB<\/jats:styled-content>). The strain provided by Fungal Genetic Stock centre was studied about the apoptotic\u2010like phenotype treated with different concentrations of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and <jats:styled-content style=\"fixed-case\">AMB<\/jats:styled-content>, and then analyzed by fluorescent microscopy (observed by Annexin\u2010V\/<jats:styled-content style=\"fixed-case\">FITC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TUNEL<\/jats:styled-content> staining), flow cytometry (stained with <jats:styled-content style=\"fixed-case\">DHR<\/jats:styled-content>123\/<jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>), and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> agarose gel electrophores. When <jats:italic>R. arrhizus<\/jats:italic> was treated with H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and <jats:styled-content style=\"fixed-case\">AMB<\/jats:styled-content>, there was a loss of viability associated with different phenotype of apoptosis makers. Membrane externalization of phosphatidylserine (<jats:styled-content style=\"fixed-case\">PS<\/jats:styled-content>) on the cell surface, <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> fragmentation, chromatin condensation can be induced and observed obviously by Annexin\u2010V\/<jats:styled-content style=\"fixed-case\">FITC<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> DAPI<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TUNEL<\/jats:styled-content> staining. <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> smear not <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> ladder was also visible in <jats:italic>R. arrhizus<\/jats:italic>. Flowcytometry of <jats:italic>R. arrhizus<\/jats:italic> cells revealed not only the increase of apoptosis cell stained with <jats:styled-content style=\"fixed-case\">DHR<\/jats:styled-content>123 under the nonfungicida doses but dead cells stained with <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content> under the fungicida concentrations.This study indicated that both H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and <jats:styled-content style=\"fixed-case\">AMB<\/jats:styled-content> could induce the apoptotic\u2010like phenotype in <jats:italic>R. arrhizus<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/myc.12240","type":"journal-article","created":{"date-parts":[[2014,9,30]],"date-time":"2014-09-30T06:35:45Z","timestamp":1412058945000},"page":"25-30","source":"Crossref","is-referenced-by-count":9,"title":["Apoptotic\u2010like phenotype triggered by hydrogen peroxide and amphotericin B in the fungus <i>Rhizopus arrhizus<\/i>"],"prefix":"10.1111","volume":"57","author":[{"given":"Sibu","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Dermatology Peking University First Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruoyu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology Peking University First Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Peking University First Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,9,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001432-200412000-00003"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.2.236"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.3.556-569.2005"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2007.01382.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01136-07"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.145.4.757"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26830-0"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.2.553-560.2006"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1087-1845(03)00047-1"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/11.19.2243"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.64.3.503-514.2000"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1224"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780600835765"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-30809-1_7"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2004.10.012"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306998200"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2332326100"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04976.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/fgbi.1998.1093"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0063393"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.2000.1716"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.1.163-177.2003"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01551-08"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-9-2409"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-4680(00)00053-5"},{"key":"e_1_2_7_27_1","first-page":"803","article-title":"Baicalein 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