{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:56:27Z","timestamp":1776300987284,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"s3","license":[{"start":{"date-parts":[[2014,9,3]],"date-time":"2014-09-03T00:00:00Z","timestamp":1409702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Mucormycosis has emerged as a relatively common severe mycosis in patients with haematological and allogeneic stem cell transplantation. Source of transmission is from unidentified sources in the environment. Early diagnosis of infection and its source of contamination are paramount for rapid and appropriate therapy. In this study, rolling circle amplification (<jats:styled-content style=\"fixed-case\">RCA<\/jats:styled-content>) is introduced as a sensitive, specific and reproducible isothermal <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> amplification technique for rapid molecular identification of six of the most virulent species (<jats:italic>Rhizopus microsporus<\/jats:italic>,<jats:italic> R.\u00a0arrhizus<\/jats:italic> var. <jats:italic>arrhizus<\/jats:italic>,<jats:italic> R.\u00a0arrhizus<\/jats:italic> var. <jats:italic>delemar<\/jats:italic>,<jats:italic> Mucor irregularis<\/jats:italic>,<jats:italic> Mucor circinelloides, Lichtheimia ramosa, Lichtheimia corymbifera<\/jats:italic>). <jats:styled-content style=\"fixed-case\">DNAs<\/jats:styled-content> of target species were successfully amplified, with no cross reactivity between species. <jats:styled-content style=\"fixed-case\">RCA<\/jats:styled-content> can be considered as a rapid detection method with high specificity and sensitivity, suitable for large screening.<\/jats:p>","DOI":"10.1111\/myc.12245","type":"journal-article","created":{"date-parts":[[2014,9,3]],"date-time":"2014-09-03T08:39:50Z","timestamp":1409733590000},"page":"67-72","source":"Crossref","is-referenced-by-count":20,"title":["Rapid screening for human\u2010pathogenic Mucorales using rolling circle amplification"],"prefix":"10.1111","volume":"57","author":[{"given":"S.","family":"Dolatabadi","sequence":"first","affiliation":[{"name":"CBS\u2010KNAW Fungal Biodiversity Centre Utrecht the Netherlands"},{"name":"Institute for Biodiversity and Ecosystem Dynamics University of Amsterdam Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. J.","family":"Najafzadeh","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology Ghaem Hospital School of Medicine Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. S.","family":"de Hoog","sequence":"additional","affiliation":[{"name":"CBS\u2010KNAW Fungal Biodiversity Centre Utrecht the Netherlands"},{"name":"Institute for Biodiversity and Ecosystem Dynamics University of Amsterdam Amsterdam the Netherlands"},{"name":"Sun Yat\u2010sen Memorial Hospital Sun Yat\u2010sen University Guangzhou China"},{"name":"Peking University Health Science Center Research Center for Medical Mycology Beijing China"},{"name":"Shanghai Institute of Medical Mycology Changzheng Hospital Second Military Medical University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,9,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.37.100183.003043"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf048147n"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03456.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir865"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir869"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir868"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir867"},{"key":"e_1_2_7_9_1","first-page":"S35","article-title":"Mucormycosis in organ and stem cell transplant recipients","volume":"54","author":"Laternier F","year":"2012","journal-title":"Clin Infect Dis"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3767\/003158513X665539"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01744-09"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3767\/003158513X665070"},{"issue":"3","key":"e_1_2_7_13_1","article-title":"Infections due to Mucormycetes: prevention and infection control","volume":"57","author":"Binder U","year":"2014","journal-title":"Mycoses"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/13693780903311944"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1086\/595846"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00056-10"},{"key":"e_1_2_7_17_1","first-page":"338","article-title":"Detection and identification of opportunistic Exophiala species using rolling circle amplification of ribosomal internal transcribed spacers","volume":"94","author":"Najafzadeh MJ","year":"2013","journal-title":"J Med Microbiol"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2011.02017.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05280-11"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13225-013-0229-6"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00420-08"},{"issue":"3","key":"e_1_2_7_22_1","article-title":"Species boundaries and nomenclature of Rhizopus arrhizus (syn. R. oryzae)","volume":"57","author":"Dolatabadi S","year":"2014","journal-title":"Mycoses"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gng104"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmyc.12245","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.12245","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T02:35:58Z","timestamp":1694572558000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.12245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,3]]},"references-count":22,"journal-issue":{"issue":"s3","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["10.1111\/myc.12245"],"URL":"https:\/\/doi.org\/10.1111\/myc.12245","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,3]]}}}