{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T18:36:18Z","timestamp":1789583778952,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,2,27]],"date-time":"2015-02-27T00:00:00Z","timestamp":1424995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Epidemiological data on the aetiologic agents of feline sporotrichosis in Malaysia have not been reported, though human sporotrichosis in Malaysia is reported to be transmitted primarily via cat scratch. To the best of our knowledge, the present report is the first study of the molecular epidemiology of <jats:italic>Sporothrix schenckii<\/jats:italic> isolates from cats with sporotrichosis in Malaysia. In the present work, we characterised 18 clinical isolates from cats in Malaysia based on molecular properties, including sequence analyses of the calmodulin gene and the <jats:styled-content style=\"fixed-case\">rDNA ITS<\/jats:styled-content> region and selective <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> of mating type (<jats:styled-content style=\"fixed-case\">MAT<\/jats:styled-content>) loci. In this study, isolates from feline sporotrichosis were identified as a <jats:italic>S. schenckii sensu stricto<\/jats:italic> by sequence analyses of the calmodulin gene and the internal transcribed spacer (<jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content>) region. Notably, phylogenetic analysis of the <jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content> confirmed assignment to clinical clade D (and not C) of <jats:italic>S. schenckii sensu stricto<\/jats:italic>. Therefore, clinical clade D of <jats:italic>S. schenckii sensu stricto<\/jats:italic> appeared to be the prevailing source of feline sporotrichosis in Malaysia. The ratio of <jats:italic><jats:styled-content style=\"fixed-case\">MAT<\/jats:styled-content>1\u20101\u20101:<jats:styled-content style=\"fixed-case\">MAT<\/jats:styled-content>1\u20102\u20101<\/jats:italic> in these Malaysian isolates was found to be 1\u00a0:\u00a00. This result suggested that a clonal strain of <jats:italic>S. schenckii<\/jats:italic> is the prevailing causative agent of feline sporotrichosis in Malaysia.<\/jats:p>","DOI":"10.1111\/myc.12302","type":"journal-article","created":{"date-parts":[[2015,2,27]],"date-time":"2015-02-27T12:48:23Z","timestamp":1425041303000},"page":"220-224","source":"Crossref","is-referenced-by-count":55,"title":["Molecular typing of <i>Sporothrix schenckii<\/i> isolates from cats in Malaysia"],"prefix":"10.1111","volume":"58","author":[{"given":"Rui","family":"Kano","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology Nihon University College of Bioresource Sciences Fujisawa Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miki","family":"Okubo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology Nihon University College of Bioresource Sciences Fujisawa Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han Hock","family":"Siew","sequence":"additional","affiliation":[{"name":"Han Veterinary Surgery Johor Bahru Johor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Kamata","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology Nihon University College of Bioresource Sciences Fujisawa Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuhiko","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Teikyo University Institute of Medical Mycology Hachioji Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00808-07"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780801992837"},{"key":"e_1_2_7_4_1","first-page":"153","article-title":"Global ITS diversity in the Sporothrix schenckii complex","volume":"66","author":"Zhou X","year":"2014","journal-title":"Fungal Divers"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2012.719648"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1112.040891"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/emi.2014.33"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002281"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2011.05229.x"},{"key":"e_1_2_7_10_1","first-page":"707","volume-title":"Medical Mycology","author":"Kwon\u2010Chung KJ","year":"1992"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.5.2092-2103.2005"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-011-9420-z"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_2_7_14_1","first-page":"357","article-title":"TREEVIEW: an application to display phylogenetic trees on personal computers","volume":"12","author":"Page RDM","year":"1996","journal-title":"Comput Appl Biosci"},{"key":"e_1_2_7_15_1","article-title":"The MAT1\u20101\/MAT1\u20102 ratio of Sporothrix globosa isolates in Japan","author":"Kano R","year":"2014","journal-title":"Mycopathologia"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.12226"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00153-14"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/00362178485380231"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a112628"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-011-9487-6"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmyc.12302","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.12302","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T20:05:35Z","timestamp":1696277135000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.12302"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,27]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["10.1111\/myc.12302"],"URL":"https:\/\/doi.org\/10.1111\/myc.12302","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,27]]}}}