{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T18:36:20Z","timestamp":1789583780887,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,2,27]],"date-time":"2015-02-27T00:00:00Z","timestamp":1424995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"VIAC UDEM","award":["UIN14035"],"award-info":[{"award-number":["UIN14035"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Chromoblastomycosis is a chronic granulomatous disease caused frequently by fungi of the <jats:italic>Fonsecaea<\/jats:italic> genus. The objective of this study was the phenotypic and molecular identification of <jats:italic>F.\u00a0pedrosoi<\/jats:italic> strains isolated from chromoblastomycosis patients in Mexico and Venezuela. Ten strains were included in this study. For phenotypic identification, we used macroscopic and microscopic morphologies, carbohydrate assimilation test, urea hydrolysis, cixcloheximide tolerance, proteolitic activity and the thermotolerance test. The antifungal activity of five drugs was evaluated against the isolates. Molecular identification was performed by sequencing the internal transcribed spacer (<jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content>) ribosomal <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> regions of the isolated strains. The physiological analysis and morphological features were variable and the precise identification was not possible. All isolates were susceptible to itraconazole, terbinafine, voriconazole and posaconazole. Amphotericin B was the least effective drug. The alignment of the 559\u2010nucleotide <jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content> sequences from our strains compared with sequences of GenBank revealed high homology with <jats:italic>F.\u00a0pedrosoi<\/jats:italic> (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"DDBJ\/EMBL\/GenBank\" xlink:href=\"EU285266.1\">EU285266.1<\/jats:ext-link>). In this study, all patients were from rural areas, six from Mexico and four from Venezuela. Ten isolates were identified by phenotypic and molecular analysis, using <jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content> sequence and demonstrated that nine isolates from Mexico and Venezuela were 100% homologous and one isolate showed a small genetic distance.<\/jats:p>","DOI":"10.1111\/myc.12308","type":"journal-article","created":{"date-parts":[[2015,2,27]],"date-time":"2015-02-27T12:57:17Z","timestamp":1425041837000},"page":"267-272","source":"Crossref","is-referenced-by-count":15,"title":["Phenotypic and molecular identification of <i>Fonsecaea pedrosoi<\/i> strains isolated from chromoblastomycosis patients in Mexico and Venezuela"],"prefix":"10.1111","volume":"58","author":[{"given":"O.","family":"Carolina Rojas","sequence":"first","affiliation":[{"name":"Divisi\u00f3n Ciencias de la Salud Departamento de Ciencias B\u00e1sicas Universidad de Monterrey San Pedro Garza Garc\u00eda Nuevo Le\u00f3n M\u00e9xico"},{"name":"Departamento de Microbiolog\u00eda Facultad de Medicina Universidad Aut\u00f3noma de Nuevo Le\u00f3n Monterrey Nuevo Le\u00f3n M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael B. R.","family":"Le\u00f3n\u2010Cach\u00f3n","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n Ciencias de la Salud Departamento de Ciencias B\u00e1sicas Universidad de Monterrey San Pedro Garza Garc\u00eda Nuevo Le\u00f3n M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Al\u00ed","family":"P\u00e9rez\u2010Maya","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Medicina Molecular Facultad de Medicina Universidad Aut\u00f3noma de Nuevo Le\u00f3n Monterrey Nuevo Le\u00f3n M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelino","family":"Aguirre\u2010Garza","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n Ciencias de la Salud Departamento de Ciencias B\u00e1sicas Universidad de Monterrey San Pedro Garza Garc\u00eda Nuevo Le\u00f3n M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda G.","family":"Moreno\u2010Trevi\u00f1o","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n Ciencias de la Salud Departamento de Ciencias B\u00e1sicas Universidad de Monterrey San Pedro Garza Garc\u00eda Nuevo Le\u00f3n M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria M.","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda Facultad de Medicina Universidad Aut\u00f3noma de Nuevo Le\u00f3n Monterrey Nuevo Le\u00f3n M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0190-9622(91)70292-A"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1996.55.45"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006957415346"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0507.2001.00613.x"},{"key":"e_1_2_7_6_1","volume-title":"Atlas of Clinical Fungi","author":"De Hoog GS","year":"2000"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780410001661464"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/13693780903503081"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041512"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3109\/13693780903008813"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780500220373"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2007.01383.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780802527178"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13225-013-0246-5"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780802538001"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"2565","DOI":"10.1128\/jcm.27.11.2565-2569.1989","article-title":"Evaluation of the API 20C yeast identification system for the differentiation of some dematiaceous fungi","volume":"27","author":"Espinel\u2010Ingroff A","year":"1989","journal-title":"J Clin Microbiol"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/02681219580000661"},{"key":"e_1_2_7_18_1","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1128\/jcm.26.2.301-307.1988","article-title":"Evaluation of proteolytic activity to differentiate some dematiaceous fungi","volume":"26","author":"Espinel\u2010Ingroff A","year":"1988","journal-title":"J Clin Microbiol"},{"key":"e_1_2_7_19_1","volume-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi; Approved Standard","author":"CLSI","year":"2008"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2012.695457"},{"key":"e_1_2_7_21_1","first-page":"315","volume-title":"PCR Protocols: A Guide to Methods and Applications","author":"White TJ","year":"1990"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2010.01937.x"},{"key":"e_1_2_7_23_1","unstructured":"1988 W.B. 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