{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T19:57:22Z","timestamp":1702670242625},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,3,10]],"date-time":"2015-03-10T00:00:00Z","timestamp":1425945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Instituto Nacional de Infectologia Evandro Chagas Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro, Brasil","award":["INI-FIOCRUZ"],"award-info":[{"award-number":["INI-FIOCRUZ"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Cryptococcus neoformans<\/jats:italic> var. <jats:italic>grubii <\/jats:italic><jats:styled-content style=\"fixed-case\">AFLP<\/jats:styled-content>1\/<jats:styled-content style=\"fixed-case\">VNI<\/jats:styled-content> is the main causative agent of cryptococcosis associated with <jats:styled-content style=\"fixed-case\">AIDS<\/jats:styled-content> in the world. <jats:italic>Cryptococcus gattii <\/jats:italic><jats:styled-content style=\"fixed-case\">AFLP<\/jats:styled-content>6\/<jats:styled-content style=\"fixed-case\">VGII<\/jats:styled-content> causes mainly endemic primary infection in immunocompetent hosts. To determine the minimum inhibitory concentrations (<jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>s) of <jats:italic>C.\u00a0neoformans<\/jats:italic> var. <jats:italic>grubii <\/jats:italic><jats:styled-content style=\"fixed-case\">AFLP<\/jats:styled-content>1\/<jats:styled-content style=\"fixed-case\">VNI<\/jats:styled-content> and <jats:italic>C.\u00a0gattii <\/jats:italic><jats:styled-content style=\"fixed-case\">AFLP<\/jats:styled-content>6\/<jats:styled-content style=\"fixed-case\">VGII<\/jats:styled-content> against amphotericin B (<jats:styled-content style=\"fixed-case\">AMB<\/jats:styled-content>) in a short period of time, flow cytometry (<jats:styled-content style=\"fixed-case\">FCM<\/jats:styled-content>) with <jats:styled-content style=\"fixed-case\">FUN<\/jats:styled-content>\u20101 fluorochrome was used to compare with broth microdilution method (<jats:styled-content style=\"fixed-case\">CLSI<\/jats:styled-content> M27\u2010A3). The minimum incubation period was evaluated by minimum fungicidal concentration procedure. Seventeen clinical isolates of <jats:italic>C.\u00a0neoformans<\/jats:italic> var. <jats:italic>grubii <\/jats:italic><jats:styled-content style=\"fixed-case\">AFLP<\/jats:styled-content>1\/<jats:styled-content style=\"fixed-case\">VNI<\/jats:styled-content> and 18 of <jats:italic>C.\u00a0gattii <\/jats:italic><jats:styled-content style=\"fixed-case\">AFLP<\/jats:styled-content>6\/<jats:styled-content style=\"fixed-case\">VGII<\/jats:styled-content> were analysed. The time for the determination of <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>s by <jats:styled-content style=\"fixed-case\">FCM<\/jats:styled-content> was 2\u00a0h against 72\u00a0h by <jats:styled-content style=\"fixed-case\">CLSI<\/jats:styled-content> M27\u2010A3 and the comparison of <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content> showed a positive significant correlation (<jats:italic>P\u00a0<\/jats:italic>=\u00a00.048). It is important to highlight the role of the <jats:styled-content style=\"fixed-case\">FCM<\/jats:styled-content> as an alternative method to determine the <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>s for <jats:styled-content style=\"fixed-case\">AMB<\/jats:styled-content> in within a day, with positive cost\u2010benefit.<\/jats:p>","DOI":"10.1111\/myc.12310","type":"journal-article","created":{"date-parts":[[2015,3,10]],"date-time":"2015-03-10T12:05:46Z","timestamp":1425989146000},"page":"273-279","source":"Crossref","is-referenced-by-count":4,"title":["<i>In vitro<\/i> susceptibility testing of amphotericin B for <i>Cryptococcus neoformans<\/i> variety <i>grubii <\/i><scp>AFLP<\/scp>1\/<scp>VN<\/scp>I and <i>Cryptococcus gattii <\/i><scp>AFLP<\/scp>6\/<scp>VG<\/scp>II by <scp>CLSI<\/scp> and flow cytometry"],"prefix":"10.1111","volume":"58","author":[{"given":"Bernardina Penarrieta","family":"Morales","sequence":"first","affiliation":[{"name":"Laboratory 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