{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T03:44:05Z","timestamp":1775879045172,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,3,24]],"date-time":"2015-03-24T00:00:00Z","timestamp":1427155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Previous reports on the molecular characteristics of clinical isolates of <jats:italic>Cryptococcus<\/jats:italic> species in China have focused on isolates from southeast China. To obtain a more detailed molecular epidemiology, a total of 92 cryptococcal isolates were collected from Sichuan province. A total of 24 isolates from 12 other provinces were collected for comparative study. Genotypes and mating types of 116 <jats:italic>Cryptococcus<\/jats:italic> isolates were determined. Among the 116 isolates, 43 isolates (19 isolates from Sichuan and 24 isolates outside of Sichuan) were analysed by multi\u2010locus sequence typing (<jats:styled-content style=\"fixed-case\">MLST<\/jats:styled-content>). All 116 clinical isolates were mating type \u03b1. Most isolates (114\/116) were molecular type <jats:styled-content style=\"fixed-case\">VNI<\/jats:styled-content> and the remaining two isolates were <jats:styled-content style=\"fixed-case\">VGI<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">VGII<\/jats:styled-content> respectively. <jats:styled-content style=\"fixed-case\">MLST<\/jats:styled-content> results revealed five sequence types (<jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>s) of <jats:italic>C. neoformans<\/jats:italic> including two novel <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>s, with most isolates identified as <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>5. The two <jats:italic>C. gattii<\/jats:italic> isolates identified in our study were <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>44 and <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>159. Based on our report and previous studies, there are 15 <jats:italic>C. neoformans <\/jats:italic><jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>s in China which can be divided into three subgroups. The <jats:italic>C. gattii<\/jats:italic> isolate from Sichuan could be a scattered subtype of <jats:styled-content style=\"fixed-case\">VGII<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>44). Our findings demonstrated that <jats:italic>C. neoformans<\/jats:italic> isolates in Sichuan are genetically homogeneous, and <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>5 is the epidemic clone of <jats:italic>C. neoformans<\/jats:italic> in China.<\/jats:p>","DOI":"10.1111\/myc.12312","type":"journal-article","created":{"date-parts":[[2015,3,24]],"date-time":"2015-03-24T21:38:43Z","timestamp":1427233123000},"page":"280-287","source":"Crossref","is-referenced-by-count":36,"title":["Molecular characterisation of clinical <i>Cryptococcus neoformans<\/i> and <i>Cryptococcus gattii<\/i> isolates from Sichuan province, China"],"prefix":"10.1111","volume":"58","author":[{"given":"Si\u2010Ying","family":"Wu","sequence":"first","affiliation":[{"name":"Division of Clinical Microbiology Department of Laboratory Medicine West China Hospital Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Lei","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology Department of Laboratory Medicine West China Hospital Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Kang","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology Department of Laboratory Medicine West China Hospital Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Ling","family":"Xiao","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology Department of Laboratory Medicine West China Hospital Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi\u2010Xing","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology Department of Laboratory Medicine West 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