{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T02:34:23Z","timestamp":1774924463218,"version":"3.50.1"},"reference-count":9,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,5,25]],"date-time":"2015-05-25T00:00:00Z","timestamp":1432512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2015,7]]},"abstract":"Summary<\/jats:title>Human histoplasmosis is caused by the dimorphic fungus Histoplasma capsulatum<\/jats:italic>. This infection can run asymptomatic or be life\u2010threatening, depending fundamentally on the host's immune status. Immunocompromised patients can present disseminated disease to the skin, making the biopsy an accessible approach. The current diagnosis gold standard is fungal culture which takes several days or weeks to grow and must be handled in a biosafe laboratory which is avoided if we use the technique here described. We propose the use of molecular biology for diagnosis confirmation, considering it can shorten diagnosis lapse, has good specificity and sensitivity and reduces the risk of infection for the medical and laboratory personnel. Seven paraffin\u2010embedded skin biopsy samples were included from patients with confirmed HIV<\/jats:styled-content> and histoplasmosis diagnosis. Total DNA<\/jats:styled-content> was isolated and molecular typing of H. capsulatum<\/jats:italic> var. capsulatum<\/jats:italic>. All samples were positive. This is a safe and accurate method for skin histoplasmosis diagnosis.<\/jats:p>","DOI":"10.1111\/myc.12337","type":"journal-article","created":{"date-parts":[[2015,5,25]],"date-time":"2015-05-25T20:14:24Z","timestamp":1432584864000},"page":"413-415","source":"Crossref","is-referenced-by-count":6,"title":["Histoplasmosis and skin lesions in HIV<\/scp>: a safe and accurate diagnosis"],"prefix":"10.1111","volume":"58","author":[{"given":"Gabriela","family":"Moreno\u2010Couti\u00f1o","sequence":"first","affiliation":[{"name":"Dermatology\/Mycology Hospital General Dr. Manuel Gea Gonz\u00e1lez Mexico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rigoberto","family":"Hern\u00e1ndez\u2010Castro","sequence":"additional","affiliation":[{"name":"Departamento de Ecologia de Agentes Patogenos Hospital General Dr. Manuel Gea Gonz\u00e1lez Mexico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Toussaint\u2010Caire","sequence":"additional","affiliation":[{"name":"Dermatopathology Hospital General Dr. Manuel Gea Gonz\u00e1lez Mexico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melisa","family":"Montiel\u2010Robles","sequence":"additional","affiliation":[{"name":"Pathogen agents Hospital General Dr. Manuel Gea Gonz\u00e1lez Mexico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fiama Selene","family":"S\u00e1nchez\u2010P\u00e9rez","sequence":"additional","affiliation":[{"name":"Dermatology Hospital General Dr. Manuel Gea Gonz\u00e1lez Mexico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Xicohtencatl\u2010Cort\u00e9s","sequence":"additional","affiliation":[{"name":"Infectolog\u00eda Hospital General Dr. Manuel Gea Gonz\u00e1lez Mexico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,5,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.13-0117"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822006000100001"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2005.73.576"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002638"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1128\/JCM.37.5.1404-1408.1999","article-title":"Relatedness analyses of Histoplasma capsulatum isolates from Mexican patients with AIDS\u2010associated histoplasmosis by using histoplasmin electrophoretic profiles and randomly amplified polymorphic DNA patterns","volume":"37","author":"Reyes Montes MR","year":"1999","journal-title":"J Clin Microbiol"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.riam.2011.03.008"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.1.205-216.2005"},{"key":"e_1_2_6_9_1","first-page":"119","article-title":"Histoplasmosis in HIV\u2010infected patients: a review of new developments and remaining gaps","volume":"1","author":"Adenis AA","year":"2014","journal-title":"Curr Trop Med Rep."},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1086\/345724"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmyc.12337","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.12337","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T22:18:45Z","timestamp":1696198725000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.12337"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,25]]},"references-count":9,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2015,7]]}},"alternative-id":["10.1111\/myc.12337"],"URL":"https:\/\/doi.org\/10.1111\/myc.12337","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,25]]}}}