{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T14:03:32Z","timestamp":1790431412145,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,7,27]],"date-time":"2015-07-27T00:00:00Z","timestamp":1437955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Non\u2010sporulating moulds (<jats:styled-content style=\"fixed-case\">NSM<\/jats:styled-content>s) isolated from respiratory specimens are usually discarded without further testing although they may have pathogenic effects in immunocompromised patients. The objective of this study was to determine the identity and frequency of <jats:styled-content style=\"fixed-case\">NSM<\/jats:styled-content>s in patients with haematological malignancies. We analysed the mycological results of 251 consecutive respiratory samples from 104 haematology patients. Yeast and sporulating moulds were identified at the genus\/species level according to their phenotypic features. <jats:styled-content style=\"fixed-case\">NSM<\/jats:styled-content>s were identified by internal transcribed spacer (<jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content>) sequencing. We detected 179 positive samples, of which 10.1% (18\/179) were mixtures of moulds and 26.3% (47\/179) were mixtures of moulds and yeast. We identified 142 moulds belonging to 11 different genera\/species or groups, with <jats:italic>Aspergillus fumigatus<\/jats:italic> (<jats:italic>n<\/jats:italic>\u00a0=\u00a050), <jats:italic>Penicillium<\/jats:italic> spp. (<jats:italic>n<\/jats:italic>\u00a0=\u00a031) and <jats:styled-content style=\"fixed-case\">NSM<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u00a0=\u00a024) being the most frequently isolated species. Twenty\u2010two <jats:styled-content style=\"fixed-case\">NSM<\/jats:styled-content>s were successfully sequenced: 18 were basidiomycetes and six were ascomycetes, corresponding to 16 different genera\/species. <jats:styled-content style=\"fixed-case\">NSM<\/jats:styled-content>s were isolated with <jats:italic>A.\u00a0fumigatus<\/jats:italic> in the same sample or in a subsequent sample in five patients with probable invasive aspergillosis. The conclusion is that the respiratory specimens of immunocompromised patients frequently contain very diverse mould species that may increase the virulence of pathogenic species. Reporting all mould species isolated when diagnosing invasive fungal infection could test this hypothesis.<\/jats:p>","DOI":"10.1111\/myc.12356","type":"journal-article","created":{"date-parts":[[2015,7,27]],"date-time":"2015-07-27T14:24:47Z","timestamp":1438007087000},"page":"557-564","source":"Crossref","is-referenced-by-count":14,"title":["High diversity of non\u2010sporulating moulds in respiratory specimens of immunocompromised patients: should all the species be reported when diagnosing invasive aspergillosis?"],"prefix":"10.1111","volume":"58","author":[{"given":"Dea","family":"Garcia\u2010Hermoso","sequence":"first","affiliation":[{"name":"Institut Pasteur Molecular Mycology Unit National Reference Center of Invasive Mycoses and Antifungals Paris France"},{"name":"CNRS URA3012 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Alanio","sequence":"additional","affiliation":[{"name":"Institut Pasteur Molecular Mycology Unit National 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