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Of the 14 <jats:italic>Malassezia<\/jats:italic> species identified, <jats:italic>M. restricta<\/jats:italic> is the most abundant fungal species found from both healthy and diseased skin. Emerging evidences have suggested that extracellular lipases of <jats:italic>Malassezia<\/jats:italic> play a critical role in its survival on the host skin surface. This study aimed to characterise the lipase 1 homologue (MrLip1) in <jats:italic>M. restricta<\/jats:italic> and to analyse its expression under different environmental conditions. The full sequence of the gene encoding MrLip1 was determined by rapid amplification of <jats:styled-content style=\"fixed-case\">cDNA<\/jats:styled-content> ends, and it was then heterologously expressed in <jats:italic>Pichia pastoris<\/jats:italic>. MrLip1 protein was successfully purified and used for lipase assay and specific antibody generation for use in expression analysis. The optimum <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> and temperature for the activity of purified MrLip1 were <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> 5.0 and 34\u00a0\u00b0C respectively. Furthermore, the expression of MrLip1 peaked at a similar <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> and temperature, suggesting that the optimal conditions for MrLip1 protein activity and expression are similar to that found on the human skin surface. This study provides data to improve our understanding of the role and characteristics of lipase 1 in <jats:italic>M. restricta<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/myc.12412","type":"journal-article","created":{"date-parts":[[2015,9,25]],"date-time":"2015-09-25T07:23:24Z","timestamp":1443165804000},"page":"671-678","source":"Crossref","is-referenced-by-count":17,"title":["Characterisation and Expression Analysis of MrLip1, a Class 3 Family Lipase of <i>Malassezia restricta<\/i>"],"prefix":"10.1111","volume":"58","author":[{"given":"Minji","family":"Park","sequence":"first","affiliation":[{"name":"Department of Systems Biotechnology Chung\u2010Ang University Anseong Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won Hee","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Systems Biotechnology Chung\u2010Ang University Anseong Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song 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