{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T18:24:31Z","timestamp":1788978271269,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,10,8]],"date-time":"2015-10-08T00:00:00Z","timestamp":1444262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"School of Medicine, Mazandaran University of Medical Sciences","award":["201232"],"award-info":[{"award-number":["201232"]}]},{"name":"Pasteur Institute"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>An increasing number of reports have described the emergence of acquired resistance of <jats:italic>Aspergillus fumigatus<\/jats:italic> to azole compounds. The primary mechanism of resistance in clinical isolates is the mutation of the azole drug target enzyme, which is encoded by the <jats:italic>cyp51A<\/jats:italic> gene. The aim of this study was to evaluate the impact of silencing the <jats:italic>cyp51A<\/jats:italic> gene in azole\u2010resistant <jats:italic>A. fumigatus<\/jats:italic> isolates. A 21\u2010nucleotide small\u2010interfering <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>) was designed based on the <jats:styled-content style=\"fixed-case\">cDNA<\/jats:styled-content> sequence of the <jats:italic>A. fumigatus cyp51A<\/jats:italic> gene. After silencing the <jats:italic>cyp51A<\/jats:italic> gene in germinated conidia (15, 20, 25 and 50 nM), azole\u2010resistant <jats:italic>A. fumigatus<\/jats:italic> was cultured on broth media and gene expression was analysed by measuring the <jats:italic>cyp51A <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> level using <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assay. Hyphae were successfully transfected by si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and expression of the <jats:italic>cyp51A<\/jats:italic> gene was significantly reduced by si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> at the concentration of 50 nM (<jats:italic>P<\/jats:italic> \u2264 0.05). In addition, at this si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> concentration, the minimum inhibitory concentration of itraconazole for the treated cells was decreased, compared with that for untreated control cells, from 16 to 4 \u03bcg\/ml.<\/jats:p>","DOI":"10.1111\/myc.12417","type":"journal-article","created":{"date-parts":[[2015,10,8]],"date-time":"2015-10-08T13:05:37Z","timestamp":1444309537000},"page":"699-706","source":"Crossref","is-referenced-by-count":15,"title":["<i>cyp51A<\/i> gene silencing using <scp>RNA<\/scp> interference in azole\u2010resistant <i><scp>A<\/scp>spergillus fumigatus<\/i>"],"prefix":"10.1111","volume":"58","author":[{"given":"Bita","family":"Mousavi","sequence":"first","affiliation":[{"name":"Department of Medical Mycology and parasitology\/Invasive Fungi Research Center School of Medicine Mazandaran University of Medical Sciences Sari Sari Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad T.","family":"Hedayati","sequence":"additional","affiliation":[{"name":"Department of Medical Mycology and parasitology\/Invasive Fungi Research Center School of Medicine Mazandaran University of Medical Sciences Sari Sari Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ladan","family":"Teimoori\u2010Toolabi","sequence":"additional","affiliation":[{"name":"Molecular Medicine Department Biotechnology Research Center Pasteur Institute of Iran Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Guillot","sequence":"additional","affiliation":[{"name":"ENVA UPEC Dynamyc Research Group Ecole nationale veterinaire d'Alfort University Paris Est Creteil Maisons Alfort Creteil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahad","family":"Alizadeh","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Reproductive Health Reproductive Epidemiology Research Center Royan Institute for 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