{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T06:08:35Z","timestamp":1774505315550,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,10,26]],"date-time":"2015-10-26T00:00:00Z","timestamp":1445817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Chronic subcutaneous infections caused by <jats:italic>Aspergillus<\/jats:italic> species are considered to be extremely rare. Because these fungi are among the most common laboratory contaminants, their role as eumycetoma causative agents is difficult to ascertain. Here, we report the first case of <jats:italic>A. flavus<\/jats:italic> eumycetoma confirmed by isolation, molecular identification and immunohistochemical analysis. Patient was a 55\u2010year\u2010old male from Sudan suffering from eumycetoma on his left foot for a period of 17\u00a0years. He developed swelling, sinuses and white grain discharge was observed. He has been operated nine times and was treated with several regimens of ketoconazole and itraconazole without improvement. Initial diagnosis based on histology and radiology was <jats:italic>Scedosporium<\/jats:italic> eumycetoma. However, examination of the biopsy revealed <jats:italic>A. flavus<\/jats:italic>, which was identified by molecular analysis and <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF MS<\/jats:styled-content>. Immunohistochemistry using antibody directed against <jats:italic>Aspergillus<\/jats:italic> species was positive. Because of the earlier treatment failures with ketoconazole and itraconazole, therapy with voriconazole was initiated. However, <jats:italic>in\u00a0vitro<\/jats:italic> susceptibility testing yielded a lower Minimum Inhibitory Concentration (MIC) value for itraconazole (0.25\u00a0\u03bcg\u00a0ml<jats:sup>\u22121<\/jats:sup>) than for voriconazole (1\u00a0\u03bcg\u00a0ml<jats:sup>\u22121<\/jats:sup>). Based on the presented results, <jats:italic>A. flavus<\/jats:italic> can be considered as one of the agents of white\u2010grain eumycetoma.<\/jats:p>","DOI":"10.1111\/myc.12422","type":"journal-article","created":{"date-parts":[[2015,10,26]],"date-time":"2015-10-26T06:44:52Z","timestamp":1445841892000},"page":"728-734","source":"Crossref","is-referenced-by-count":19,"title":["Seventeen years of subcutaneous infection by <i>Aspergillus flavus<\/i>; eumycetoma confirmed by immunohistochemistry"],"prefix":"10.1111","volume":"58","author":[{"given":"Sarah A.","family":"Ahmed","sequence":"first","affiliation":[{"name":"Faculty of Medical Laboratory Sciences University of Khartoum Khartoum Sudan"},{"name":"CBS\u2010KNAW Fungal Biodiversity Centre Utrecht The Netherlands"},{"name":"Institute for Biodiversity and Ecosystem Dynamics University of Amsterdam Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manal A.","family":"Abbas","sequence":"additional","affiliation":[{"name":"Mycology Laboratory Faculty of Medicine University of Khartoum Khartoum Sudan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory","family":"Jouvion","sequence":"additional","affiliation":[{"name":"Human Histopathology and Animal Models Unit Institute Pasteur Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdullah M. 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Sybren","family":"de Hoog","sequence":"additional","affiliation":[{"name":"CBS\u2010KNAW Fungal Biodiversity Centre Utrecht The Netherlands"},{"name":"Institute for Biodiversity and Ecosystem Dynamics University of Amsterdam Amsterdam The Netherlands"},{"name":"Basic Pathology Department Federal University of Paran\u00e1 Curitiba Brazil"},{"name":"Biological Sciences Department Faculty of Science King Abdulaziz University Jeddah Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Kolecka","sequence":"additional","affiliation":[{"name":"CBS\u2010KNAW Fungal Biodiversity Centre Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"El Sheikh","family":"Mahgoub","sequence":"additional","affiliation":[{"name":"Mycetoma Research Centre University of Khartoum Khartoum 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