{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T06:17:33Z","timestamp":1783577853505,"version":"3.55.0"},"reference-count":55,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,10,26]],"date-time":"2015-10-26T00:00:00Z","timestamp":1445817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The increasing incidence of invasive fungal diseases (<jats:styled-content style=\"fixed-case\">IFD<\/jats:styled-content>), most of all invasive aspergillosis (<jats:styled-content style=\"fixed-case\">IA<\/jats:styled-content>) in immunocompromised patients emphasises the need to improve the diagnostic tools for detection of fungal pathogens. We investigated the diagnostic performance of a multifungal <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010microarray detecting 15 different fungi [<jats:italic>Aspergillus, Candida, Fusarium, Mucor, Rhizopus, Scedosporium<\/jats:italic> and <jats:italic>Trichosporon<\/jats:italic> species (spp.)] in addition to an <jats:italic>Aspergillus<\/jats:italic> specific polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) assay. Biopsies, bronchoalveolar lavage and peripheral blood samples of 133 immunocompromised patients (pts) were investigated by a multifungal <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010microarray as well as a nested <jats:italic>Aspergillus<\/jats:italic> specific <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assay. Patients had proven (<jats:italic>n<\/jats:italic>\u00a0=\u00a018), probable (<jats:italic>n<\/jats:italic>\u00a0=\u00a029), possible (<jats:italic>n<\/jats:italic>\u00a0=\u00a048) and no <jats:styled-content style=\"fixed-case\">IFD<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u00a0=\u00a038) and were mostly under antifungal therapy at the time of sampling. The results were compared to culture, histopathology, imaging and serology, respectively. For the non\u2010<jats:italic>Aspergillus <\/jats:italic><jats:styled-content style=\"fixed-case\">IFD<\/jats:styled-content> the microarray analysis yielded in all samples a sensitivity of 64% and a specificity of 80%. Best results for the detection of all <jats:styled-content style=\"fixed-case\">IFD<\/jats:styled-content> were achieved by combining <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010microarray and <jats:italic>Aspergillus<\/jats:italic> specific <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> in biopsy samples (sensitivity 79%; specificity 71%). The molecular assays in combination identify genomic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> of fungal pathogens and may improve identification of causative pathogens of <jats:styled-content style=\"fixed-case\">IFD<\/jats:styled-content> and help overcoming the diagnostic uncertainty of culture and\/or histopathology findings, even during antifungal therapy.<\/jats:p>","DOI":"10.1111\/myc.12424","type":"journal-article","created":{"date-parts":[[2015,10,26]],"date-time":"2015-10-26T08:15:25Z","timestamp":1445847325000},"page":"735-745","source":"Crossref","is-referenced-by-count":38,"title":["Identification of invasive fungal diseases in immunocompromised patients by combining an <i>Aspergillus<\/i> specific <scp>PCR<\/scp> with a multifungal <scp>DNA<\/scp>\u2010microarray from primary clinical samples"],"prefix":"10.1111","volume":"58","author":[{"given":"T.","family":"Boch","sequence":"first","affiliation":[{"name":"Department of Hematology and Oncology University Hospital Mannheim University of Heidelberg Mannheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Reinwald","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology University Hospital Mannheim University of Heidelberg Mannheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Postina","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology University Hospital Mannheim University of Heidelberg Mannheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O. A.","family":"Cornely","sequence":"additional","affiliation":[{"name":"Department I of Internal Medicine University Hospital of Cologne Cologne Germany"},{"name":"Clinical Trials Centre Cologne ZKS K\u00f6ln, BMBF 01KN1106 Centre for Integrated Oncology CIO K\u00f6ln Bonn Cologne Germany"},{"name":"Cologne Excellence Cluster on Cellular Stress Response in Aging\u2010Associated Diseases (CECAD) University of Cologne Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. J.","family":"Vehreschild","sequence":"additional","affiliation":[{"name":"Department I of Internal Medicine University Hospital of Cologne Cologne Germany"},{"name":"German Centre for Infection Research Partner site Bonn\u2010Cologne Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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