{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:25:18Z","timestamp":1740126318422,"version":"3.37.3"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,11,18]],"date-time":"2015-11-18T00:00:00Z","timestamp":1447804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003545","name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Inova\u00e7\u00e3o","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003545","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Histoplasmosis is a systemic fungal disease that occurs worldwide, causing symptomatic infection mostly in immunocompromised hosts. Etiological agent is the dimorphic fungus, <jats:italic>Histoplasma capsulatum,<\/jats:italic> which occurs in soil contaminated with bird or bat droppings. Major limitation in recognition of <jats:italic>H. capsulatum<\/jats:italic> infections is the low awareness, since other diseases may have similar symptomatology. The molecular methods have gained importance because of unambiguous diagnostic ability and efficiency. The aim of this study was to develop and evaluate a padlock probe in view of rolling circle amplification (<jats:styled-content style=\"fixed-case\">RCA<\/jats:styled-content>) detection method which targets ITS (Internal Transcribed Spacer) <jats:styled-content style=\"fixed-case\">rDNA<\/jats:styled-content> of <jats:italic>H. capsulatum<\/jats:italic> enabling rapid and specific detection of the fungus in clinical samples. Two padlock probes were designed and one of these (Hc<jats:styled-content style=\"fixed-case\">PL<\/jats:styled-content>2) allowed specific amplification of <jats:italic>H. capsulatum<\/jats:italic> <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> while no cross\u2010reactivity was observed with fungi used as negative controls. This method proved to be effective for <jats:italic>H. capsulatum<\/jats:italic> specific identification and demonstrated to be faster than the traditional method of microbiological identification.<\/jats:p>","DOI":"10.1111\/myc.12426","type":"journal-article","created":{"date-parts":[[2015,11,18]],"date-time":"2015-11-18T11:02:58Z","timestamp":1447844578000},"page":"12-19","source":"Crossref","is-referenced-by-count":12,"title":["Molecular identification of <i><scp>H<\/scp>istoplasma capsulatum<\/i> using rolling circle amplification"],"prefix":"10.1111","volume":"59","author":[{"given":"Jason L.","family":"Furuie","sequence":"first","affiliation":[{"name":"Bioprocess Engineering and Biotechnology Department Post\u2010Graduation Program of Bioprocess Engineering and Biotechnology Federal University of Paran\u00e1 Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiufeng","family":"Sun","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Institute of Public Health Guangdong Provincial Center for Disease Control and Prevention Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana M. F.","family":"do Nascimento","sequence":"additional","affiliation":[{"name":"Basic Pathology Department Post\u2010Graduation Program of Microbiology, Parasitology and Pathology Federal University of Paran\u00e1 Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata R.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Basic Pathology Department Post\u2010Graduation Program of Microbiology, Parasitology and Pathology Federal University of Paran\u00e1 Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline E.","family":"Waculicz\u2010Andrade","sequence":"additional","affiliation":[{"name":"Basic Pathology Department Post\u2010Graduation Program of Microbiology, Parasitology and Pathology Federal University of Paran\u00e1 Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gisele C.","family":"Sessegolo","sequence":"additional","affiliation":[{"name":"Ecossistema Environmental Consulting Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0891-4563","authenticated-orcid":false,"given":"Anderson M.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Cellular Biology Division Microbiology, Immunology and Parasitology Department Federal University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria A.","family":"Galv\u00e3o\u2010Dias","sequence":"additional","affiliation":[{"name":"Center for Zoonosis Control S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoilo P.","family":"de Camargo","sequence":"additional","affiliation":[{"name":"Cellular Biology Division Microbiology, Immunology and Parasitology Department Federal University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1vio","family":"Queiroz\u2010Telles","sequence":"additional","affiliation":[{"name":"Basic Pathology Department Post\u2010Graduation Program of Microbiology, Parasitology and Pathology Federal University of Paran\u00e1 Curitiba Brazil"},{"name":"Clinical Hospital of the Federal University of Paran\u00e1 Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad J.","family":"Najafzadeh","sequence":"additional","affiliation":[{"name":"Parasitology and Mycology Department Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sybren G.","family":"de Hoog","sequence":"additional","affiliation":[{"name":"Basic Pathology Department Post\u2010Graduation Program of Microbiology, Parasitology and Pathology Federal University of Paran\u00e1 Curitiba Brazil"},{"name":"CBS\u2010KNAW Fungal Biodiversity Centre Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vania A.","family":"Vicente","sequence":"additional","affiliation":[{"name":"Bioprocess Engineering and Biotechnology Department Post\u2010Graduation Program of Bioprocess Engineering and Biotechnology Federal University of Paran\u00e1 Curitiba Brazil"},{"name":"Basic Pathology Department Post\u2010Graduation Program of Microbiology, Parasitology and Pathology Federal University of Paran\u00e1 Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,11,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0037-86822011000100007"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.55.6.1355-1358.1987"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1906.62510440037003"},{"key":"e_1_2_6_5_1","first-page":"342","volume-title":"Manuale de micologia m\u00e9dica","author":"Ciferri R","year":"1960"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2307\/3793310"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.riam.2013.10.003"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46651992000600018"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccm.2009.02.002"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00204-10"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rce.2013.07.014"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0037-86822009000200020"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652007000100007"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.3.653-663.1999"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294X.2003.01995.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.funbio.2011.12.004"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.535-539.2003"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02739-13"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-10-264"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-006-0207-y"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-012-9567-2"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.riam.2013.03.004"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-002-0833-y"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2013.06.026"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00420-08"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200700903"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02235-07"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.5.2339-2344.2005"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0034-89102010005000004"},{"key":"e_1_2_6_30_1","first-page":"315","volume-title":"PCR Protocols: A Guide to Methods and Applications","author":"White TJ","year":"1990"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2011.02017.x"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbn013"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.1.77-84.2006"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05280-11"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02898-12"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2012.01.017"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1039\/C1AN15886D"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmyc.12426","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.12426","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T13:08:02Z","timestamp":1696165682000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.12426"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,18]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["10.1111\/myc.12426"],"URL":"https:\/\/doi.org\/10.1111\/myc.12426","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"type":"print","value":"0933-7407"},{"type":"electronic","value":"1439-0507"}],"subject":[],"published":{"date-parts":[[2015,11,18]]}}}