{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T12:45:09Z","timestamp":1717764309708},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,11,23]],"date-time":"2015-11-23T00:00:00Z","timestamp":1448236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Laboratory of Infectious Diseases Unit"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The purpose of this study was to analyse specific molecular mechanisms involved in the intrinsic resistance of <jats:italic>C. albicans<\/jats:italic> biofilms to antifungals. We investigated the transcriptional profile of three genes (<jats:italic><jats:styled-content style=\"fixed-case\">BGL<\/jats:styled-content>2, <jats:styled-content style=\"fixed-case\">SUN<\/jats:styled-content>41, <jats:styled-content style=\"fixed-case\">ECE<\/jats:styled-content>1<\/jats:italic>) involved in <jats:italic>Candida<\/jats:italic> cell wall formation in response to voriconazole or anidulafungin after the production of intermediate and mature biofilms. <jats:italic>C. albicans<\/jats:italic> M61, a well\u2010documented biofilm producer strain, was used for the development of intermediate (12 h and 18 h) and completely mature biofilms (48 h). After exposure of cells from each biofilm growth mode to voriconazole (128 and 512 mg l<jats:sup>\u22121<\/jats:sup>) or anidulafungin (0.25 and 1 mg l<jats:sup>\u22121<\/jats:sup>) for 12\u201324 h, total <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> samples extracted from biofilm cells were analysed by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. The voriconazole and anidulafungin biofilm <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content> was 512 and 0.5 mg l<jats:sup>\u22121<\/jats:sup> respectively. Anidulafungin caused significant up\u2010regulation of <jats:italic><jats:styled-content style=\"fixed-case\">SUN<\/jats:styled-content>41<\/jats:italic> (3.7\u20139.3\u2010fold) and <jats:italic><jats:styled-content style=\"fixed-case\">BGL<\/jats:styled-content>2<\/jats:italic> (2.2\u20132.8 fold) in intermediately mature biofilms; whereas, voriconazole increased gene expression in completely mature biofilms (<jats:italic><jats:styled-content style=\"fixed-case\">SUN<\/jats:styled-content>41<\/jats:italic> 2.3\u2010fold, <jats:italic><jats:styled-content style=\"fixed-case\">BGL<\/jats:styled-content>2<\/jats:italic> 2.1\u2010fold). Gene expression was primarily down\u2010regulated by voriconazole in intermediately, but not completely mature biofilms. Both antifungals caused down\u2010regulation of <jats:italic><jats:styled-content style=\"fixed-case\">ECE<\/jats:styled-content>1<\/jats:italic> in intermediately mature biofilms.<\/jats:p>","DOI":"10.1111\/myc.12436","type":"journal-article","created":{"date-parts":[[2015,11,23]],"date-time":"2015-11-23T05:38:47Z","timestamp":1448257127000},"page":"43-47","source":"Crossref","is-referenced-by-count":2,"title":["Differential effects of antifungal agents on expression of genes related to formation of <i>Candida albicans<\/i> biofilms"],"prefix":"10.1111","volume":"59","author":[{"given":"Athanasios","family":"Chatzimoschou","sequence":"first","affiliation":[{"name":"Infectious Diseases Laboratory 3rd Department of Pediatrics Faculty of Medicine Hippokration Hospital Aristotle University School of Health Sciences Thessaloniki Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Simitsopoulou","sequence":"additional","affiliation":[{"name":"Infectious Diseases Laboratory 3rd Department of Pediatrics Faculty of Medicine Hippokration Hospital Aristotle University School of Health Sciences Thessaloniki Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charalampos","family":"Antachopoulos","sequence":"additional","affiliation":[{"name":"Infectious Diseases Laboratory 3rd Department of Pediatrics Faculty of Medicine Hippokration Hospital Aristotle University School of Health Sciences Thessaloniki Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas J.","family":"Walsh","sequence":"additional","affiliation":[{"name":"Transplantation\u2010Oncology Infectious Diseases Program Departments of Medicine, Pediatrics, and Microbiology &amp; Immunology Weill Cornell Medical Center of Cornell University New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel","family":"Roilides","sequence":"additional","affiliation":[{"name":"Infectious Diseases Laboratory 3rd Department of Pediatrics Faculty of Medicine Hippokration Hospital Aristotle University School of Health Sciences Thessaloniki Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,11,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.045054-0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-015-0083-3"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2011.02076.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.5812\/jjm.17105"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2911(08)60307-0"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsps.2013.12.007"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.6.2226-2236.2005"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01638-09"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.61.9.3648-3655.1993"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00163-12"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00314-07"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00959-10"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(02)00002-1"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2008.01569.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00856-07"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.2.878-888.2002"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.030692-0"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2006.10.003"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.18.5385-5394.2001"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02290-12"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.4.501"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002848"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14472-8"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728214.11.2.231"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmyc.12436","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.12436","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T13:07:52Z","timestamp":1696165672000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.12436"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,23]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["10.1111\/myc.12436"],"URL":"https:\/\/doi.org\/10.1111\/myc.12436","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,23]]}}}