{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:09:34Z","timestamp":1761581374872,"version":"3.37.3"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,12,10]],"date-time":"2015-12-10T00:00:00Z","timestamp":1449705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["2U2RTW007367"],"award-info":[{"award-number":["2U2RTW007367"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Cryptococcal meningitis is the leading fungal infection and <jats:styled-content style=\"fixed-case\">AIDS<\/jats:styled-content> defining opportunistic illness in patients with late stage <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content> infection, particularly in South\u2010East Asia and sub\u2010Saharan Africa. Given the high mortality, clinical differences and the extensive ecological niche of <jats:italic>Cryptococcus neoformans<\/jats:italic> and <jats:italic>Cryptococcus gattii<\/jats:italic> species complexes, there is need for laboratories in sub\u2010Sahara African countries to adopt new and alternative reliable diagnostic algorithms that rapidly identify and distinguish these species. We biotyped 74 and then amplified fragment length polymorphism (<jats:styled-content style=\"fixed-case\">AFLP<\/jats:styled-content>) genotyped 66 <jats:italic>Cryptococcus<\/jats:italic> isolates from a cohort of patients with <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010associated cryptococcal meningitis. <jats:italic>C. gattii<\/jats:italic> sensu lato was isolated at a prevalence of 16.7% (<jats:italic>n<\/jats:italic> = 11\/66) and <jats:italic>C<\/jats:italic>. <jats:italic>neoformans<\/jats:italic> sensu stricto was responsible for 83.3% (<jats:italic>n<\/jats:italic> = 55\/66) of the infections. <jats:sc>l<\/jats:sc>\u2010Canavanine glycine bromothymol blue, yeast\u2010carbon\u2010base\u2010<jats:sc>d<\/jats:sc>\u2010proline\u2010<jats:sc>d<\/jats:sc>\u2010tryptophan and creatinine dextrose bromothymol blue thymine were able to distinguish pathogenic <jats:italic>C<\/jats:italic>. <jats:italic>gattii<\/jats:italic> sensu lato from <jats:italic>C<\/jats:italic>.<jats:italic> neoformans<\/jats:italic> sensu stricto species when compared with AFLP genotyping. This study demonstrates high <jats:italic>C<\/jats:italic>. <jats:italic>gattii<\/jats:italic> sensu lato prevalence in Zimbabwe. In addition, biotyping methods can be used as alternative diagnostic tools to molecular typing in resource\u2010limited areas for differentiating pathogenic <jats:italic>Cryptococcus<\/jats:italic> species.<\/jats:p>","DOI":"10.1111\/myc.12444","type":"journal-article","created":{"date-parts":[[2015,12,10]],"date-time":"2015-12-10T21:10:34Z","timestamp":1449781834000},"page":"151-156","source":"Crossref","is-referenced-by-count":12,"title":["Comparison of biotyping methods as alternative identification tools to molecular typing of pathogenic <i><scp>C<\/scp>ryptococcus<\/i> species in sub\u2010Saharan Africa"],"prefix":"10.1111","volume":"59","author":[{"given":"Tinashe K.","family":"Nyazika","sequence":"first","affiliation":[{"name":"Department of Chemical Pathology College of Health Sciences University of Zimbabwe Harare Zimbabwe"},{"name":"Department of Medical Microbiology College of Health Sciences University of Zimbabwe Harare 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Netherlands"},{"name":"Department of Medical Microbiology Radboudumc Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5622-1916","authenticated-orcid":false,"given":"Ferry","family":"Hagen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases Canisius\u2010Wilhelmina Hospital Nijmegen The 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