{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:51:18Z","timestamp":1790718678418,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,1,25]],"date-time":"2016-01-25T00:00:00Z","timestamp":1453680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Onychomycosis is a common nail disorder mainly due to dermatophytes for which the conventional diagnosis requires direct microscopic observation and culture of a biological sample. Nevertheless, antifungal treatments are commonly prescribed without a mycological examination having been performed, partly because of the slow growth of dermatophytes. Therefore, molecular biology has been applied to this pathology, to support a quick and accurate distinction between onychomycosis and other nail damage. Commercial kits are now available from several companies for improving traditional microbiological diagnosis. In this paper, we present the first evaluation of the real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> kit marketed by Bio Evolution for the diagnosis of dermatophytosis. Secondly, we compare the efficacy of the kit on optimal and non\u2010optimal samples. This study was conducted on 180 nails samples, processed by conventional methods and retrospectively analysed using this kit. According to our results, this molecular kit has shown high specificity and sensitivity in detecting dermatophytes, regardless of sample quality. On the other hand, and as expected, optimal samples allowed the identification of a higher number of dermatophytes by conventional mycological diagnosis, compared to non\u2010optimal samples. Finally, we have suggested several strategies for the practical use of such a kit in a medical laboratory for quick pathogen detection.<\/jats:p>","DOI":"10.1111\/myc.12471","type":"journal-article","created":{"date-parts":[[2016,1,25]],"date-time":"2016-01-25T07:20:42Z","timestamp":1453706442000},"page":"304-311","source":"Crossref","is-referenced-by-count":40,"title":["Optimising the diagnostic strategy for onychomycosis from sample collection to <scp>FUNGAL<\/scp> identification evaluation of a diagnostic kit for real\u2010time <scp>PCR<\/scp>"],"prefix":"10.1111","volume":"59","author":[{"given":"Dimitri","family":"Petinataud","sequence":"first","affiliation":[{"name":"Structure de Parasitologie\u2010Mycologie D\u00e9partement de Microbiologie Centre Hospitalo\u2010Universitaire de Nancy (CHU\u2010Nancy) H\u00f4pitaux de Brabois Vand\u0153uvre\u2010les\u2010Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sibel","family":"Berger","sequence":"additional","affiliation":[{"name":"Plateforme de g\u00e9nomique microbienne D\u00e9partement de Microbiologie CHU\u2010Nancy H\u00f4pitaux de Brabois Vand\u0153uvre\u2010les\u2010Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cyril","family":"Ferdynus","sequence":"additional","affiliation":[{"name":"Service de Biostatistiques CHU F\u00e9lix Guyon la R\u00e9union Centre de M\u00e9thodologie et de Gestion des donn\u00e9es: Unit\u00e9 de Soutien M\u00e9thodologique CHU La R\u00e9union\u2013Site du CHFG Route de Bellepierre Saint Denis Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Debourgogne","sequence":"additional","affiliation":[{"name":"Structure de Parasitologie\u2010Mycologie D\u00e9partement de Microbiologie Centre Hospitalo\u2010Universitaire de Nancy (CHU\u2010Nancy) H\u00f4pitaux de Brabois Vand\u0153uvre\u2010les\u2010Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nelly","family":"Contet\u2010Audonneau","sequence":"additional","affiliation":[{"name":"Structure de Parasitologie\u2010Mycologie D\u00e9partement de Microbiologie Centre Hospitalo\u2010Universitaire de Nancy (CHU\u2010Nancy) H\u00f4pitaux de Brabois Vand\u0153uvre\u2010les\u2010Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Machouart","sequence":"additional","affiliation":[{"name":"Structure de Parasitologie\u2010Mycologie D\u00e9partement de Microbiologie Centre Hospitalo\u2010Universitaire de Nancy (CHU\u2010Nancy) H\u00f4pitaux de Brabois Vand\u0153uvre\u2010les\u2010Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,1,25]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"1222","article-title":"Can we evaluate the frequency of 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