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Recently, non\u2010<jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected hosts were increasingly reported to form a considerable proportion. However, the majority of the reported studies on the diagnosis of CM patients were performed on <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected patients. For evaluation of various diagnostic approaches for <jats:styled-content style=\"fixed-case\">CM<\/jats:styled-content> in non\u2010<jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected patients, a range of conventional and molecular assays used for diagnosis of <jats:styled-content style=\"fixed-case\">CM<\/jats:styled-content> were verified on 85 clinical <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>s from non\u2010<jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected <jats:styled-content style=\"fixed-case\">CM<\/jats:styled-content> patients, including India ink staining, culture, a newly developed loop\u2010mediated isothermal amplification (<jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content>), the lateral flow assay (<jats:styled-content style=\"fixed-case\">LFA<\/jats:styled-content>) of cryptococcal antigen detection and a <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> assay. The <jats:styled-content style=\"fixed-case\">LFA<\/jats:styled-content> had the highest positive detection rate (97.6%; 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>, 91.8\u201399.7%) in non\u2010<jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected <jats:styled-content style=\"fixed-case\">CM<\/jats:styled-content> patients, followed by the <jats:styled-content style=\"fixed-case\">LAMP<\/jats:styled-content> (87.1%; 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>, 78.0\u201393.4%), the <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> (80.0%; 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>, 69.9\u201387.9%), India ink staining (70.6%; 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>, 59.7\u201380.0%) and culture (35.3%; 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>, 25.2\u201346.4%). All culture positive specimens were correctly identified by the LFA.<\/jats:p>","DOI":"10.1111\/myc.12497","type":"journal-article","created":{"date-parts":[[2016,4,10]],"date-time":"2016-04-10T18:26:25Z","timestamp":1460312785000},"page":"494-502","source":"Crossref","is-referenced-by-count":25,"title":["Evaluation of five conventional and molecular approaches for diagnosis of cryptococcal meningitis in non\u2010<scp>HIV<\/scp>\u2010infected patients"],"prefix":"10.1111","volume":"59","author":[{"given":"Min","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Dermatology Shanghai Key Laboratory of Molecular Medical Mycology Shanghai Institute of Medical Mycology Changzheng Hospital Second Military Medical University Shanghai China"},{"name":"CBS\u2010KNAW Fungal Biodiversity Centre Utrecht the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Dermatology Shanghai Seventh People's Hospital Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology Shanghai Key Laboratory of Molecular Medical Mycology Shanghai Institute of Medical Mycology Changzheng Hospital Second Military Medical University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology Shanghai Key Laboratory of Molecular Medical Mycology Shanghai Institute of Medical Mycology Changzheng Hospital Second Military Medical University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pharmacy General Hospital of Jinan Military Command Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen J.","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Shanghai Key Laboratory of Molecular Medical Mycology Shanghai Institute of Medical Mycology Changzheng Hospital Second Military Medical University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdullah M. 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