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Looking for alternatives, this study evaluated antifungal agents, alone and combined, correlating susceptibility to genotypes. Eighty Cryptococcus<\/jats:italic> clinical isolates were genotyped by URA<\/jats:styled-content><\/jats:italic>5 gene restriction fragment length polymorphism. Antifungal susceptibility was assessed following CLSI<\/jats:styled-content>\u2010M27A3 for amphotericin (AMB<\/jats:styled-content>), 5\u2010flucytosine (5FC<\/jats:styled-content>), fluconazole (FCZ<\/jats:styled-content>), voriconazole (VRZ<\/jats:styled-content>), itraconazole (ITZ<\/jats:styled-content>) and terbinafine (TRB<\/jats:styled-content>). Drug interaction chequerboard assay evaluated: AMB<\/jats:styled-content> + 5FC<\/jats:styled-content>, AMB<\/jats:styled-content> + FCZ<\/jats:styled-content>, AMB<\/jats:styled-content> + TRB<\/jats:styled-content> and FCZ<\/jats:styled-content> + TRB<\/jats:styled-content>. Molecular typing divided isolates into 14 C. deuterogattii<\/jats:italic> (VGII<\/jats:styled-content>) and C. neoformans<\/jats:italic> isolates were found to belong to genotype VNI<\/jats:styled-content> (n<\/jats:italic> = 62) and VNII<\/jats:styled-content> (n<\/jats:italic> = 4). C. neoformans <\/jats:italic>VNII<\/jats:styled-content> was significantly less susceptible than VNI<\/jats:styled-content> (P<\/jats:italic> = 0.0407) to AMB<\/jats:styled-content>; C. deuterogattii<\/jats:italic> was significantly less susceptible than VNI<\/jats:styled-content> and VNII<\/jats:styled-content> to VRZ<\/jats:styled-content> (P<\/jats:italic> < 0.0001). C. deuterogattii<\/jats:italic> was less susceptible than C. neoformans <\/jats:italic>VNI<\/jats:styled-content> for FCZ<\/jats:styled-content> (P<\/jats:italic> = 0.0170), ITZ<\/jats:styled-content> (P<\/jats:italic> < 0.0001) and TRB<\/jats:styled-content> (P<\/jats:italic> = 0.0090). The combination FCZ<\/jats:styled-content> + TRB<\/jats:styled-content> showed 95.16% of synergistic effect against C. neoformans<\/jats:italic> genotype VNI<\/jats:styled-content> isolates and all combinations showed 100% of synergism against genotype VNII<\/jats:styled-content> isolates, suggesting the relevance of cryptococcal genotyping as it is widely known that the various genotypes (now species) have significant impact in antifungal susceptibilities and clinical outcome. In difficult\u2010to\u2010treat cryptococcosis, terbinafine and different antifungal combinations might be alternatives to 5FC<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/myc.12510","type":"journal-article","created":{"date-parts":[[2016,5,2]],"date-time":"2016-05-02T15:32:13Z","timestamp":1462203133000},"page":"585-593","source":"Crossref","is-referenced-by-count":14,"title":["Evaluation of antifungal combination against Cryptococcus<\/i> spp."],"prefix":"10.1111","volume":"59","author":[{"given":"Franqueline","family":"Reichert\u2010Lima","sequence":"first","affiliation":[{"name":"Clinical Pathology Department Faculty of Medical Sciences State University of Campinas Campinas Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariane F.","family":"Busso\u2010Lopes","sequence":"additional","affiliation":[{"name":"Internal Medicine Department Faculty of Medical Sciences State University of Campinas Campinas Sao Paulo 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