{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T11:57:18Z","timestamp":1769601438845,"version":"3.49.0"},"reference-count":27,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,7,12]],"date-time":"2016-07-12T00:00:00Z","timestamp":1468281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Dermatological Society of Thailand"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Knowledge regarding host immune response to chromoblastomycosis and eumycetoma is limited, particularly concerning cytokines and antimicrobial peptides production. This was a retrospective study of 12 paraffin\u2010embedded tissue samples from patients diagnosed with chromoblastomycosis or eumycetoma from histological findings and tissue culture. <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> extraction and polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) from tissues were done to evaluate human interleukin\u201017A (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A), interferon\u2010gamma (<jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3), tumour necrosis factor\u2010alpha (<jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1), interleukin\u20101 beta (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2) and human beta\u2010defensin\u20102 (<jats:styled-content style=\"fixed-case\">HBD<\/jats:styled-content>\u20102) expressions. Human beta\u2010actin primer was used for confirming <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> detection, and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> extracted from psoriasis lesional skin samples was used as positive controls. The twelve paraffin\u2010embedded sections used in this study consisted of five chromoblastomycosis and seven eumycetoma tissues. All <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> reactions showed beta\u2010actin band at 51 bp in all clinical specimens, confirming adequate <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> levels in each reaction. As positive control, the psoriasis skin samples revealed bands for <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A at 174 bp, <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 at 273 bp, <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 at 360 bp, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2 at 276 bp and <jats:styled-content style=\"fixed-case\">HBD<\/jats:styled-content>\u20102 at 255 bp. For the chromoblastomycosis and eumycetoma tissues, <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> analyses showed <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A band at 174 bp in two eumycetoma tissues and <jats:styled-content style=\"fixed-case\">HBD<\/jats:styled-content>\u20102 band at 255 bp in a chromoblastomycosis tissue. This study demonstrated <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A expression in human eumycetoma and <jats:styled-content style=\"fixed-case\">HBD<\/jats:styled-content>\u20102 expression in human chromoblastomycosis for the first time. However, their role in immune response remains to be elucidated.<\/jats:p>","DOI":"10.1111\/myc.12526","type":"journal-article","created":{"date-parts":[[2016,7,12]],"date-time":"2016-07-12T08:35:28Z","timestamp":1468312528000},"page":"751-756","source":"Crossref","is-referenced-by-count":6,"title":["Immune response in human chromoblastomycosis and eumycetoma \u2013 focusing on human interleukin\u201017A, interferon\u2010gamma, tumour necrosis factor\u2010alpha, interleukin\u20101 beta and human beta\u2010defensin\u20102"],"prefix":"10.1111","volume":"59","author":[{"given":"Charussri","family":"Leeyaphan","sequence":"first","affiliation":[{"name":"Department of Dermatology Graduate School of Medicine Teikyo University Tokyo Japan"},{"name":"Department of Dermatology Faculty of Medicine Siriraj Hospital, Mahidol University Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carren","family":"Hau","sequence":"additional","affiliation":[{"name":"Department 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