{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T11:10:48Z","timestamp":1784718648942,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T00:00:00Z","timestamp":1469577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Cryptococcal meningitis is a severe opportunistic infection in <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected patients. In Ivory Coast, despite the availability of antiretroviral treatment (<jats:styled-content style=\"fixed-case\">ART<\/jats:styled-content>), this infection is still prevalent. The study investigates the genetic diversity of 363 clinical isolates of <jats:italic>Cryptococcus<\/jats:italic> from 61 Ivorian <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010positive patients, the occurrence of mixed infections and the in vitro antifungal susceptibility of the isolates. Serotyping was performed <jats:italic>via <\/jats:italic><jats:styled-content style=\"fixed-case\">LAC<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">CAP<\/jats:styled-content>64 gene amplification. Genotyping was performed using the phage M13 core (<jats:styled-content style=\"fixed-case\">GACA<\/jats:styled-content>)<jats:sub>4<\/jats:sub> and (<jats:styled-content style=\"fixed-case\">GTG<\/jats:styled-content>)<jats:sub>5<\/jats:sub> primers and restriction fragment length polymorphism analysis of the <jats:italic><jats:styled-content style=\"fixed-case\">URA<\/jats:styled-content>5<\/jats:italic> gene. By <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> fingerprinting, the presence of the three serotypes were demonstrated among the 363 isolates in the population studied: A (n=318; 87.6%), <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> (n=40; 11%) and B (n=4; 1.1%). Using <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> fingerprinting with primers M13 (<jats:styled-content style=\"fixed-case\">GACA<\/jats:styled-content>)<jats:sub>4<\/jats:sub> and (<jats:styled-content style=\"fixed-case\">GTG<\/jats:styled-content>)<jats:sub>5<\/jats:sub>, we grouped the isolates into 56 molecular subtypes. We observed a high frequency (39.3%) of mixed infections, with up to two different genotypes per sample. None of the isolates were resistant to amphotericin B. Only 0.3% and 1.1% of the isolates were resistant to fluconazole and flucytosine respectively. This study revealed the high genetic diversity among <jats:italic>Cryptococcus<\/jats:italic> isolates, the occurrence of mixed infections and a high antifungal susceptibility for the majority of Ivorian cryptococcal isolates.<\/jats:p>","DOI":"10.1111\/myc.12539","type":"journal-article","created":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T08:27:59Z","timestamp":1469608079000},"page":"811-817","source":"Crossref","is-referenced-by-count":22,"title":["Molecular epidemiology reveals genetic diversity among 363 isolates of the <i>Cryptococcus neoformans<\/i> and <i>Cryptococcus gattii<\/i> species complex in 61 Ivorian <scp>HIV<\/scp>\u2010positive patients"],"prefix":"10.1111","volume":"59","author":[{"given":"Fulgence K.","family":"Kassi","sequence":"first","affiliation":[{"name":"Laboratoire de Parasitologie et de Mycologie \u2013 CeDReS (Centre de Diagnostic et de Recherche sur le SIDA et les autres maladies infectieuses) UFR Pharmacie CHU de Treichville Universit\u00e9 F\u00e9lix Houphou\u00ebt Boigny  Abidjan C\u00f4te d'Ivoire"},{"name":"UMI 233 IRD\u2010UM INSERM U1175 Laboratoire de Parasitologie et de Mycologie, UFR Pharmacie  Montpellier Cedex 5 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Drakulovski","sequence":"additional","affiliation":[{"name":"UMI 233 IRD\u2010UM INSERM U1175 Laboratoire de Parasitologie et de Mycologie, UFR Pharmacie  Montpellier Cedex 5 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Bellet","sequence":"additional","affiliation":[{"name":"UMI 233 IRD\u2010UM INSERM U1175 Laboratoire de Parasitologie et de Mycologie, UFR Pharmacie  Montpellier Cedex 5 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donika","family":"Krasteva","sequence":"additional","affiliation":[{"name":"UMI 233 IRD\u2010UM INSERM U1175 Laboratoire de Parasitologie et de Mycologie, UFR Pharmacie  Montpellier Cedex 5 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Gatchitch","sequence":"additional","affiliation":[{"name":"UMI 233 IRD\u2010UM INSERM U1175 Laboratoire de Parasitologie et de Mycologie, UFR Pharmacie  Montpellier Cedex 5 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adama","family":"Doumbia","sequence":"additional","affiliation":[{"name":"Service des Maladies Infectieuses et Tropicales CHU de Treichville  Abidjan C\u00f4te d'Ivoire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gis\u00e8le A.","family":"Kouakou","sequence":"additional","affiliation":[{"name":"Service des Maladies Infectieuses et Tropicales CHU de Treichville  Abidjan C\u00f4te d'Ivoire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Delaporte","sequence":"additional","affiliation":[{"name":"UMI 233 Service des Maladies Infectieuses et Tropicales CHU Gui de Chauliac  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Reynes","sequence":"additional","affiliation":[{"name":"UMI 233 Service des Maladies Infectieuses et Tropicales CHU Gui de Chauliac  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mich\u00e8le","family":"Malli\u00e9","sequence":"additional","affiliation":[{"name":"UMI 233 IRD\u2010UM INSERM U1175 Laboratoire de Parasitologie et de Mycologie, UFR Pharmacie  Montpellier Cedex 5 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9 I. 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