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Nine cases of mycetoma were analysed in a retrospective study. All isolates were identified by morphological features. The level of species identification was reached by molecular tools. Definitive identification of fungi was performed using sequence analysis of the <jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content> of the ribosomal <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> region and the ribosomal large\u2010subunit D1\/D2. Identification of actinomycetes was accomplished by the 16S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> gene sequence. Six unusual clinical isolates were identified: <jats:italic>Aspergillus ustus, Cyphellophora oxyspora, Exophiala oligosperma, Madurella pseudomycetomatis<\/jats:italic>,<jats:italic> Nocardia farcinica<\/jats:italic> and <jats:italic>Nocardia wallacei<\/jats:italic>. The prevalence of mycetoma in Venezuela remains unknown. This study represents the first report in the literature of mycetoma caused by unusual pathogens identified by molecular techniques.<\/jats:p>","DOI":"10.1111\/myc.12579","type":"journal-article","created":{"date-parts":[[2016,10,21]],"date-time":"2016-10-21T05:52:57Z","timestamp":1477029177000},"page":"129-135","source":"Crossref","is-referenced-by-count":10,"title":["Molecular identification of unusual Mycetoma agents isolated from patients in Venezuela"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7717-7072","authenticated-orcid":false,"given":"Olga C.","family":"Rojas","sequence":"first","affiliation":[{"name":"Departamento de Ciencias B\u00e1sicas Divisi\u00f3n de Ciencias de la Salud Universidad de Monterrey  San Pedro Garza Garc\u00edaNuevo Le\u00f3n Mexico"},{"name":"Departamento de Microbiolog\u00eda Facultad de Medicina Universidad Aut\u00f3noma de Nuevo Le\u00f3n  Monterrey Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1639-6869","authenticated-orcid":false,"given":"Rafael B. 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