{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T01:11:27Z","timestamp":1784077887859,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T00:00:00Z","timestamp":1479254400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2017,4]]},"abstract":"Summary<\/jats:title>Limited specific data and investigations are available for the diagnosis of Invasive Fungal Infection (IFI<\/jats:styled-content>) in paediatrics cancer patients. Three non\u2010invasive tests; Platelia Aspergillus <\/jats:italic>EIA<\/jats:styled-content> for galactomannan (GM<\/jats:styled-content>), \u03b2\u2010D\u2010glucan (BDG<\/jats:styled-content>) assay and pan\u2010fungal real\u2010time PCR<\/jats:styled-content> for fungal DNA<\/jats:styled-content> in blood were evaluated. One hundred twenty\u2010five paediatrics cancer patients at the high risk of IFI<\/jats:styled-content> were enrolled. Single blood and serum samples were evaluated by all the three methods. Patients were classified into 10 proven, 52 probable and 63 no IFI<\/jats:styled-content> cases in accordance with EORTC MSG<\/jats:styled-content> 2008 revised guidelines. The sensitivity, specificity, PPV<\/jats:styled-content> and NPV<\/jats:styled-content> of all the three tests in proven, probable and no IFI<\/jats:styled-content>s cases were analysed singly and in combination. The sensitivity, specificity, PPV<\/jats:styled-content> and NPV<\/jats:styled-content> of GM<\/jats:styled-content>, BDG<\/jats:styled-content> and pan\u2010fungal real\u2010time PCR<\/jats:styled-content> were: 87%, 61%, 81%, 69.5% for GM<\/jats:styled-content>, 88%, 59.5%, 81%, 71.4% for BDG<\/jats:styled-content> and 89%, 69.2%, 85%, 67.5% for PCR<\/jats:styled-content> (95% CI<\/jats:styled-content>). Among different combinations, best combination was found to be GM<\/jats:styled-content> and PCR<\/jats:styled-content> with sensitivity, specificity, PPV<\/jats:styled-content> and NPV<\/jats:styled-content> of 98.2%, 89.3%, 97.1% and 90% respectively. Single samples must be evaluated by combination of tests.<\/jats:p>","DOI":"10.1111\/myc.12584","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T19:43:52Z","timestamp":1479325432000},"page":"234-240","source":"Crossref","is-referenced-by-count":33,"title":["Comparative evaluation of pan\u2010fungal real\u2010time PCR<\/scp>, galactomannan and (1\u20103)\u2010\u03b2\u2010D\u2010glucan assay for invasive fungal infection in paediatric cancer patients"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6925-3546","authenticated-orcid":false,"given":"Prashant","family":"Gupta","sequence":"first","affiliation":[{"name":"Department of Microbiology King George's Medical University Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abrar","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Microbiology King George's Medical University Lucknow 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