{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T14:39:27Z","timestamp":1769524767319,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,3,24]],"date-time":"2017-03-24T00:00:00Z","timestamp":1490313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Pathogenesis of cryptococcosis in the central nervous system (<jats:styled-content style=\"fixed-case\">CNS<\/jats:styled-content>) is a topic of ongoing research, including the mechanisms by which this fungus invades and infects the brain. Astrocytes, the most common <jats:styled-content style=\"fixed-case\">CNS<\/jats:styled-content> cells, play a fundamental role in the local immune response. Astrocytes might participate in cryptococcosis either as a host or by responding to fungal antigens. To determine the infectivity of <jats:italic>Cryptococcus\u00a0neoformans<\/jats:italic> var. <jats:italic>grubii<\/jats:italic> and <jats:italic>Cryptococcus\u00a0gattii<\/jats:italic> in a human astrocytoma cell line and the induction of major histocompatibility complex (<jats:styled-content style=\"fixed-case\">MHC<\/jats:styled-content>) molecules. A glioblastoma cell line was infected with <jats:italic>C.\u00a0neoformans<\/jats:italic> var. <jats:italic>grubii<\/jats:italic> and <jats:italic>C.\u00a0gattii<\/jats:italic> blastoconidia labelled with <jats:styled-content style=\"fixed-case\">FUN<\/jats:styled-content>\u20101 fluorescent stain. The percentage of infection and expression of <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content> class I and <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> molecules were determined by flow cytometry. The interactions between the fungi and cells were observed by fluorescence microscopy. There was no difference between <jats:italic>C.\u00a0neoformans<\/jats:italic> var. <jats:italic>grubii<\/jats:italic> and <jats:italic>C.\u00a0gattii<\/jats:italic> in the percentage infection, but <jats:italic>C.\u00a0neoformans<\/jats:italic> var. <jats:italic>grubii<\/jats:italic> induced higher expression of <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content> class <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> than <jats:italic>C.\u00a0gattii<\/jats:italic>. More blastoconidia were recovered from <jats:italic>C.\u00a0neoformans<\/jats:italic>\u2010infected cells than from <jats:italic>C. gattii<\/jats:italic> infected cells. <jats:italic>Cryptococcus neoformans<\/jats:italic> var. <jats:italic>grubii<\/jats:italic> may have different virulence mechanisms that allow its survival in human glia\u2010derived cells.<\/jats:p>","DOI":"10.1111\/myc.12619","type":"journal-article","created":{"date-parts":[[2017,3,24]],"date-time":"2017-03-24T16:19:11Z","timestamp":1490372351000},"page":"447-453","source":"Crossref","is-referenced-by-count":14,"title":["Infective capacity of <i>Cryptococcus neoformans<\/i> and <i>Cryptococcus gattii<\/i> in a human astrocytoma cell line"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5109-907X","authenticated-orcid":false,"given":"M. C.","family":"Olave","sequence":"first","affiliation":[{"name":"Grupo Ciencias B\u00e1sicas M\u00e9dicas Facultad de Medicina Universidad de los Andes  Bogot\u00e1 DC Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5609-7046","authenticated-orcid":false,"given":"J. C.","family":"Vargas\u2010Zambrano","sequence":"additional","affiliation":[{"name":"Grupo Ciencias B\u00e1sicas M\u00e9dicas Facultad de Medicina Universidad de los Andes  Bogot\u00e1 DC Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3057-1966","authenticated-orcid":false,"given":"A. 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M.","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Grupo Ciencias B\u00e1sicas M\u00e9dicas Facultad de Medicina Universidad de los Andes  Bogot\u00e1 DC Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,3,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e328322ffac"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2016.167"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/CLEP.S38850"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a019760"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.16-0148"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00126-13"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2522"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402981101"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1602.090900"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/fov106"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00103-12"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003272"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.15.83"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-014-0051-0"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048570"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00554-13"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-009-0619-8"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2015.08.017"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/465319"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1590\/0074-0276108022013014"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2004.07.001"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-008-0060-5"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22305"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.12.6720"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-199611000-00006"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2003.12.010"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830260231"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.2000.00683.x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.1.365"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01425-15"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20538"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(88)90738-0"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmyc.12619","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.12619","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T10:12:56Z","timestamp":1696155176000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.12619"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,24]]},"references-count":32,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1111\/myc.12619"],"URL":"https:\/\/doi.org\/10.1111\/myc.12619","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,24]]}}}