{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:51:18Z","timestamp":1790718678424,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,5,16]],"date-time":"2017-05-16T00:00:00Z","timestamp":1494892800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI14CIII\/00045"],"award-info":[{"award-number":["PI14CIII\/00045"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["FI12\/00095 and PI14CIII\/00045"],"award-info":[{"award-number":["FI12\/00095 and PI14CIII\/00045"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Onychomycosis (OM) is a nail infection caused mainly by dermatophyte species but other species of yeast and moulds are frequently involved as well. Classical diagnosis has limitations thus empirical treatment is common. The usefulness of different real time PCR (RT\u2010PCR) assays for identifying species causing OM was assessed in samples from seventy patients and fifteen controls. Conventional methods and four different RT\u2010PCR assays were used: a panfungal, a pandermatophyte and two specific assays for detecting <jats:italic>Candida<\/jats:italic> spp. and <jats:italic>Aspergillus<\/jats:italic> spp. Fungal elements were visualised in 58% of the samples, and 54% of cultures were positive. Panfungal and pandermatophyte RT\u2010PCR were positive in 28% and 60%, respectively, and the sensitivity relative to positive cultures was 47% and 90%. <jats:italic>Candida<\/jats:italic> spp. were detected in 76% of samples analysed and <jats:italic>Aspergillus<\/jats:italic> spp. in 60%. These species were also present in 80% of control cases. In conclusion, molecular techniques were useful but showed limitations. The panfungal assay showed a low sensitivity, the pandermatophyte assay was sensitive and specific but did not allow for differentiation among species of dermatophytes. Finally, the role of non\u2010dermatophyte species detected by using specific RT\u2010PCR techniques should be carefully analysed as these species were also present in healthy nails.<\/jats:p>","DOI":"10.1111\/myc.12629","type":"journal-article","created":{"date-parts":[[2017,5,16]],"date-time":"2017-05-16T07:26:42Z","timestamp":1494919602000},"page":"638-644","source":"Crossref","is-referenced-by-count":17,"title":["Usefulness of techniques based on real time PCR for the identification of onychomycosis\u2010causing species"],"prefix":"10.1111","volume":"60","author":[{"given":"Anissa Z.","family":"Hafirassou","sequence":"first","affiliation":[{"name":"Laboratory of Mycology Biotechnology and Microbial Activities Fr\u00e8res\u2010Mentouri, Constantine University  Constantine Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9923-4151","authenticated-orcid":false,"given":"Clara","family":"Valero","sequence":"additional","affiliation":[{"name":"Mycology Department Centro Nacional de Microbiolog\u00eda Instituto de Salud Carlos III  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Gassem","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology and Medical Mycology CHU Ben Badis  Constantine Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilhem","family":"Mihoubi","sequence":"additional","affiliation":[{"name":"Laboratory of Mycology Biotechnology and Microbial Activities Fr\u00e8res\u2010Mentouri, Constantine University  Constantine Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1878-6067","authenticated-orcid":false,"given":"Maria J.","family":"Buitrago","sequence":"additional","affiliation":[{"name":"Mycology Department Centro Nacional de Microbiolog\u00eda Instituto de Salud Carlos III  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004105"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cpm.2016.02.001"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2006.12.019"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2011.02.038"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.12323"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.12788\/j.sder.2016.008"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2006.01230.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2003.05242.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2015.4190"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12109"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2014.10.008"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2009.09291.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2011.09.005"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2009.09249.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12110"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2012.07.050"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku225"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00328-14"},{"key":"e_1_2_7_20_1","volume-title":"Molecular Cloning. 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