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This pilot study aimed to gain an overview of the intra\u2010 and inter\u2010individual variability in <jats:italic>Aspergillus fumigatus<\/jats:italic> reactive T\u2010helper cells in healthy adults and the correlation with environmental mould exposure. In this flow cytometric study, the frequencies of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>154<jats:sup>+<\/jats:sup> <jats:italic>A.\u00a0fumigatus<\/jats:italic> reactive T cells were evaluated in 70 healthy volunteers. All subjects completed a standardised questionnaire addressing their mould exposure. Subjects with intensive mould exposure in their professional or residential surrounding demonstrated considerably higher mean frequencies of <jats:italic>A.\u00a0fumigatus<\/jats:italic> reactive T\u2010helper and T\u2010memory cells. Comparative evaluation of multiple measurements over time demonstrated relatively conserved reactive T\u2010cell frequencies in the absence of major changes to the exposure profile, whereas those frequently exposed in professional environment or with changes to their risk score demonstrated a marked dependency of antigen reactive T\u2010cell frequencies on recent mould exposure. This pilot study was the first to provide data on the intra\u2010individual variability in <jats:italic>A.\u00a0fumigatus<\/jats:italic> reactive T\u2010cell frequencies and its linkage to mould encounter. Fungus reactive T cells are to be considered a valued tool for the assessment of environmental mould exposure.<\/jats:p>","DOI":"10.1111\/myc.12643","type":"journal-article","created":{"date-parts":[[2017,8,8]],"date-time":"2017-08-08T11:46:48Z","timestamp":1502192808000},"page":"668-675","source":"Crossref","is-referenced-by-count":13,"title":["Intra\u2010 and inter\u2010individual variability of <i>Aspergillus fumigatus<\/i> reactive T\u2010cell frequencies in healthy volunteers in dependency of mould exposure in residential and working environment"],"prefix":"10.1111","volume":"60","author":[{"given":"Sebastian","family":"Wurster","sequence":"first","affiliation":[{"name":"Department of Internal Medicine II Division of Infectious Diseases University Hospital of Wuerzburg  Wuerzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Weis","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II Division of Infectious Diseases University Hospital of Wuerzburg  Wuerzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukas","family":"Page","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II Division of Infectious Diseases University Hospital of Wuerzburg  Wuerzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Helm","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II Division of Infectious Diseases University Hospital of Wuerzburg  Wuerzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Lazariotou","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II Division of Infectious Diseases University Hospital of Wuerzburg  Wuerzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann","family":"Einsele","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II Division of Infectious Diseases University Hospital of Wuerzburg  Wuerzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8191-6517","authenticated-orcid":false,"given":"Andrew J.","family":"Ullmann","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II Division of Infectious Diseases University Hospital of Wuerzburg  Wuerzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(99)00026-7"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1128\/aem.45.5.1501-1505.1983","article-title":"Levels of gram\u2010negative bacteria, Aspergillus fumigatus, dust, and endotoxin at compost plants","volume":"45","author":"Clark CS","year":"1983","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00014-09"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2939"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-014-0722-7"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit237"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2008.02.014"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1202221"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2008.271"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu607"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2015.05.011"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-07-366526"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0074326"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0149108"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201407-1235LE"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.07.010"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myw054"},{"key":"e_1_2_7_19_1","unstructured":"WursterS HelmJ WeisP et\u00a0al.Ciclosporin A MMF and prednisolone lead to reduced sensitivity of the quantification of Aspergillus\u2010specific T\u2010cells. 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