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The objective of this study was to standardise an alternative method for the production and indirect quantification of melanin in <jats:italic>C<\/jats:italic>.\u00a0<jats:italic>neoformans sensu lato<\/jats:italic> and <jats:italic>C<\/jats:italic>.\u00a0<jats:italic>gattii sensu lato<\/jats:italic><jats:bold>.<\/jats:bold> Eight <jats:italic>C<\/jats:italic>.\u00a0<jats:italic>neoformans sensu lato<\/jats:italic> and three <jats:italic>C<\/jats:italic>.\u00a0<jats:italic>gattii sensu lato<\/jats:italic>, identified through <jats:styled-content style=\"fixed-case\">URA<\/jats:styled-content>5 methodology, <jats:italic>Candida parapsilosis <\/jats:italic><jats:styled-content style=\"fixed-case\">ATCC<\/jats:styled-content> 22019 (negative control) and one <jats:italic>Hortaea werneckii<\/jats:italic> (positive control) were inoculated on minimal medium agar with or without L\u2010<jats:styled-content style=\"fixed-case\">DOPA<\/jats:styled-content>, in duplicate, and incubated at 35\u00b0C, for 7\u00a0days. Pictures were taken from the third to the seventh day, under standardised conditions in a photographic chamber. Then, photographs were analysed using grayscale images<jats:bold>.<\/jats:bold> All <jats:italic>Cryptococcus<\/jats:italic> spp. strains produced melanin after growth on minimal medium agar containing L\u2010<jats:styled-content style=\"fixed-case\">DOPA<\/jats:styled-content>. <jats:italic>C.\u00a0parapsilosis <\/jats:italic><jats:styled-content style=\"fixed-case\">ATCC<\/jats:styled-content> 22019 did not produce melanin on medium containing L\u2010<jats:styled-content style=\"fixed-case\">DOPA<\/jats:styled-content>, while <jats:italic>H.\u00a0werneckii<\/jats:italic> presented the strongest pigmentation. This new method allows the indirect analysis of melanin production through pixel quantification in grayscale images, enabling the study of substances that can modulate melanin production.<\/jats:p>","DOI":"10.1111\/myc.12650","type":"journal-article","created":{"date-parts":[[2017,7,12]],"date-time":"2017-07-12T05:26:39Z","timestamp":1499837199000},"page":"697-702","source":"Crossref","is-referenced-by-count":27,"title":["An alternative method for the analysis of melanin production in <i>Cryptococcus neoformans sensu lato<\/i> and <i>Cryptococcus gattii sensu lato<\/i>"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5102-3582","authenticated-orcid":false,"given":"Raimunda S. 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